Continuous production of lipase-catalyzed biodiesel in a packed-bed reactor: optimization and enzyme reuse study.
An optimal continuous production of biodiesel by methanolysis of soybean oil in a packed-bed reactor was developed using immobilized lipase (Novozym 435) as a catalyst in a tert-butanol solvent system. Response surface methodology (RSM) and Box-Behnken design were employed to evaluate the effects of...
| Publicado en: | Journal of Biomedicine & Biotechnology pp. 950725 - 950726 |
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| Autores principales: | , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Wiley-Blackwell
2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104539426&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104539426 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11107243 137K jtl: Journal of Biomedicine & Biotechnology issn: 11107243 maglogo: N pubinfo: dt: 2011 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104539426 104539426 2011456850 NLM20936129 PMC2948937 104539426 ppf: 950725 ppct: 1 formats: fmt: @attributes: type: P tig: atl: Continuous production of lipase-catalyzed biodiesel in a packed-bed reactor: optimization and enzyme reuse study. aug: au: Chen, Hsiao-Ching Ju, Hen-Yi Wu, Tsung-Ta Liu, Yung-Chuan Lee, Chih-Chen Chang, Cheng Chung, Yi-Lin Shieh, Chwen-Jen affil: Department and Graduate Program of Bioindustry Technology, Dayeh University, Chang-Hua, Taiwan. sug: subj: Energy-Generating Resources Biotechnology Enzymes Metabolism Lipase Metabolism Analysis of Variance Enzymes Equipment Reuse Lipase Models, Theoretical Regression Soybean Oil Metabolism Temperature ab: An optimal continuous production of biodiesel by methanolysis of soybean oil in a packed-bed reactor was developed using immobilized lipase (Novozym 435) as a catalyst in a tert-butanol solvent system. Response surface methodology (RSM) and Box-Behnken design were employed to evaluate the effects of reaction temperature, flow rate, and substrate molar ratio on the molar conversion of biodiesel. The results showed that flow rate and temperature have significant effects on the percentage of molar conversion. On the basis of ridge max analysis, the optimum conditions were as follows: flow rate 0.1 mL/min, temperature 52.1°C, and substrate molar ratio 1 : 4. The predicted and experimental values of molar conversion were 83.31 ± 2.07% and 82.81 ± .98%, respectively. Furthermore, the continuous process over 30 days showed no appreciable decrease in the molar conversion. The paper demonstrates the applicability of using immobilized lipase and a packed-bed reactor for continuous biodiesel synthesis. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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