A murine model of Cryptococcus gattii meningoencephalitis.
Objectives: Meningoencephalitis caused by Cryptococcus gattii is associated with significant morbidity and the need for aggressive therapy, and often necessitates neurosurgical intervention. We adapted a previously described murine model of cryptococcal meningoencephalitis due to Cryptococcus neofor...
| Publicado en: | Journal of Antimicrobial Chemotherapy (JAC) Vol. 67; no. 6; pp. 1432 - 1439 |
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| Autores principales: | , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Oxford University Press / USA
Jun2012
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104567112&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104567112 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03057453 N5O jtl: Journal of Antimicrobial Chemotherapy (JAC) issn: 03057453 maglogo: N pubinfo: dt: Jun2012 vid: 67 iid: 6 pid: 622 pub: Oxford University Press / USA artinfo: ui: 104567112 NLM22378683 2011549398 10.1093/jac/dks060 NLM22378683 PMC3350330 104567112 ppf: 1432 ppct: 7 formats: tig: atl: A murine model of Cryptococcus gattii meningoencephalitis. aug: au: Thompson GR 3rd Wiederhold NP Najvar LK Bocanegra R Kirkpatrick WR Graybill JR Patterson TF Thompson, George R 3rd Wiederhold, Nathan P Najvar, Laura K Bocanegra, Rosie Kirkpatrick, William R Graybill, John R Patterson, Thomas F affil: Department of Medical Microbiology and Immunology and Department of Internal Medicine the Division of Infectious Diseases, The University of California Davis, Davis, CA, USA sug: subj: Cryptococcosis Microbiology Cryptococcosis Pathology Cryptococcus Meningoencephalitis Microbiology Meningoencephalitis Pathology Models, Biological Animal Studies Antifungal Agents Administration and Dosage Antigens, Fungal Blood Brain Microbiology Colony Count, Microbial Cryptococcosis Drug Therapy Fluconazole Administration and Dosage Heterocyclic Compounds Administration and Dosage Histocytochemistry Meningoencephalitis Drug Therapy Mice Survival Analysis ab: Objectives: Meningoencephalitis caused by Cryptococcus gattii is associated with significant morbidity and the need for aggressive therapy, and often necessitates neurosurgical intervention. We adapted a previously described murine model of cryptococcal meningoencephalitis due to Cryptococcus neoformans to that caused by C. gattii.Methods: Mice were inoculated intracranially with either C. gattii (genotype VGIIa) or C. neoformans. In virulence studies, different C. gattii infecting inocula were compared with a fixed inoculum of C. neoformans, and differences were assessed by survival, brain tissue fungal burden, serum antigen titres and histopathological changes within brain tissue. For treatment, fluconazole or posaconazole (10 mg/kg orally twice daily) was initiated 24 h post-inoculation.Results: C. gattii was more virulent than C. neoformans, as evident by shorter median survival, earlier histopathological changes and higher serum antigen titres. However, no differences in fungal burden or dissemination to other organs were observed among the various groups. In treatment studies, both fluconazole and posaconazole improved the median survival of mice infected with either species. However, neither regimen improved the percentage of animals surviving to the predetermined study endpoint.Conclusions: These results demonstrate the virulence of C. gattii meningoencephalitis and the potential of this model for the assessment of new treatment strategies. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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