Biophotonic techniques for the study of malaria-infected red blood cells.
Investigation of the homeostasis of red blood cells upon infection by Plasmodium falciparum poses complex experimental challenges. Changes in red cell shape, volume, protein, and ion balance are difficult to quantify. In this article, we review a wide range of optical techniques for quantitative mea...
| Publicado en: | Medical & Biological Engineering & Computing Vol. 48; no. 10; pp. 1055 - 1064 |
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| Autores principales: | , , , , , , , , , , , , , , , , , , , |
| Formato: | research review Journal Article |
| Publicado: |
Springer Nature
Oct2010
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104569369&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104569369 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01400118 PO0 jtl: Medical & Biological Engineering & Computing issn: 01400118 maglogo: N pubinfo: dt: Oct2010 vid: 48 iid: 10 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104569369 NLM20661776 2010799206 10.1007/s11517-010-0668-0 NLM20661776 104569369 ppf: 1055 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Biophotonic techniques for the study of malaria-infected red blood cells. aug: au: Mauritz JM Esposito A Tiffert T Skepper JN Warley A Yoon YZ Cicuta P Lew VL Guck JR Kaminski CF Mauritz, Jakob M A Esposito, Alessandro Tiffert, Teresa Skepper, Jeremy N Warley, Alice Yoon, Young-Zoon Cicuta, Pietro Lew, Virgilio L Guck, Jochen R Kaminski, Clemens F affil: Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK sug: subj: Erythrocytes Malaria Blood Diagnostic Imaging Methods Erythrocytes Pathology Erythrocytes Physiology Biological Phenomena Microsurgery Methods Lasers Protozoa Physiology ab: Investigation of the homeostasis of red blood cells upon infection by Plasmodium falciparum poses complex experimental challenges. Changes in red cell shape, volume, protein, and ion balance are difficult to quantify. In this article, we review a wide range of optical techniques for quantitative measurements of critical homeostatic parameters in malaria-infected red blood cells. Fluorescence lifetime imaging and tomographic phase microscopy, quantitative deconvolution microscopy, and X-ray microanalysis, are used to measure haemoglobin concentration, cell volume, and ion contents. Atomic force microscopy is briefly reviewed in the context of these optical methodologies. We also describe how optical tweezers and optical stretchers can be usefully applied to empower basic malaria research to yield diagnostic information on cell compliance changes upon malaria infection. The combined application of these techniques sheds new light on the detailed mechanisms of malaria infection providing potential for new diagnostic or therapeutic approaches. pubtype: Academic Journal doctype: research review Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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