Evaluation of multi-exponential curve fitting analysis of oxygen-quenched phosphorescence decay traces for recovering microvascular oxygen tension histograms.
Although it is generally accepted that oxygen-quenched phosphorescence decay traces can be analyzed using the exponential series method (ESM), its application until now has been limited to a few (patho)physiological studies, probably because the reliability of the recovered oxygen tension (pO(2)) hi...
| Publicado en: | Medical & Biological Engineering & Computing Vol. 48; no. 12; pp. 1233 - 1243 |
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| Autores principales: | , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Dec2010
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104569458&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104569458 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01400118 PO0 jtl: Medical & Biological Engineering & Computing issn: 01400118 maglogo: N pubinfo: dt: Dec2010 vid: 48 iid: 12 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104569458 NLM21046272 2010872578 10.1007/s11517-010-0698-7 NLM21046272 PMC2993890 104569458 ppf: 1233 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Evaluation of multi-exponential curve fitting analysis of oxygen-quenched phosphorescence decay traces for recovering microvascular oxygen tension histograms. aug: au: Bezemer R Faber DJ Almac E Kalkman J Legrand M Heger M Ince C Bezemer, Rick Faber, Dirk J Almac, Emre Kalkman, Jeroen Legrand, Matthieu Heger, Michal Ince, Can affil: Department of Translational Physiology, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands sug: subj: Oxygen Blood Animals Anoxia Blood Endotoxemia Blood Luminescent Measurements Methods Microcirculation Physiology Partial Pressure Rats ab: Although it is generally accepted that oxygen-quenched phosphorescence decay traces can be analyzed using the exponential series method (ESM), its application until now has been limited to a few (patho)physiological studies, probably because the reliability of the recovered oxygen tension (pO(2)) histograms has never been extensively evaluated and lacks documentation. The aim of this study was, therefore, to evaluate the use of the ESM to adequately determine pO(2) histograms from phosphorescence decay traces. For this purpose we simulated decay traces corresponding to uni- and bimodal pO(2) distributions and recovered the pO(2) histograms at different signal-to-noise ratios (SNRs). Ultimately, we recovered microvascular pO(2) histograms measured in the rat kidney in a model of endotoxemic shock and fluid resuscitation and showed that the mean microvascular oxygen tension, [Symbol: see text]pO(2)[Symbol: see text], decreased after induction of endotoxemia and that after 2 h of fluid resuscitation, [Symbol: see text]pO(2)[Symbol: see text] remained low, but the hypoxic peak that had arisen during endotoxemia was reduced. This finding illustrates the importance of recovering pO(2) histograms under (patho)physiological conditions. In conclusion, this study has characterized how noise affects the recovery of pO(2) histograms using the ESM and documented the reliability of the ESM for recovering both low- and high-pO(2) distributions for SNRs typically found in experiments. This study might therefore serve as a frame of reference for investigations focused on oxygen (re)distribution during health and disease and encourage researchers to (re-)analyze data obtained in (earlier) studies possibly revealing new insights into complex disease states and treatment strategies. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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