Transcript levels of major MMPs and ADAMTS-4 in relation to the clinicopathological profile of patients with lumbar disc herniation.
The involvement of matrix metalloproteinases (MMPs) in both the pathogenesis of intervertebral disc (ID) herniation and the spontaneous regression of herniated ID fragments remains only partially elucidated. The purpose of the present study was to simultaneously examine the transcript levels of a la...
| Publicado en: | European Spine Journal Vol. 20; no. 5; pp. 781 - 791 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
May2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104571390&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104571390 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09406719 NPI jtl: European Spine Journal issn: 09406719 maglogo: N pubinfo: dt: May2011 vid: 20 iid: 5 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104571390 NLM20857147 2011031929 10.1007/s00586-010-1573-9 NLM20857147 PMC3082673 104571390 ppf: 781 ppct: 10 formats: tig: atl: Transcript levels of major MMPs and ADAMTS-4 in relation to the clinicopathological profile of patients with lumbar disc herniation. aug: au: Tsarouhas A Soufla G Katonis P Pasku D Vakis A Spandidos DA Tsarouhas, Alexandros Soufla, Giannoula Katonis, Pavlos Pasku, Dritan Vakis, Antonis Spandidos, Demetrios A affil: Laboratory of Virology, Medical School, University of Crete, and Department of Orthopedics and Traumatology, University Hospital of Heraklion, P.O. Box 1527, 710 03, Crete, Heraklion, Greece sug: subj: Glycoproteins Intervertebral Disk Intervertebral Disk Displacement Peptide Hydrolases Adult Aged Comorbidity Trends Disease Susceptibility Female Glycoproteins Physiology Human Male Middle Age Reverse Transcriptase Polymerase Chain Reaction Methods RNA Adult: 19-44 years Aged: 65+ years Middle Aged: 45-64 years Female Male ab: The involvement of matrix metalloproteinases (MMPs) in both the pathogenesis of intervertebral disc (ID) herniation and the spontaneous regression of herniated ID fragments remains only partially elucidated. The purpose of the present study was to simultaneously examine the transcript levels of a large number of MMPs (-1, -3, -8, -9, -13 and -14) and ADAMTS-4 (a disintegrin and metalloproteinase with thrombospondin motifs) and to investigate their correlation with the clinicopathologic profile of patients suffering from symptomatic lumbar ID herniation. mRNA expression levels were determined by means of the real-time polymerase chain reaction in 63 herniated and 10 control ID specimens. Our results showed multiple positive correlations among all MMPs and ADAMTS-4 mRNA in herniated samples, indicating their possible synergistic effect in ID herniation. MMP-9 and -13 mRNA levels were significantly elevated in patients with chronic pain, presumably as a consequence of neovascularization and chronic inflammation. Smoking habits were found to have a negative dose-dependent effect on the transcript levels of MMP-3 and MMP-13 and a positive correlation with pain intensity, suggesting an unfavorable role for smoking in the regression process of herniated disc fragments. Our findings provide evidence of the molecular portrait of MMPs and ADAMTS-4 in lumbar ID herniation, as well as of its association with the clinicopathological profile of the patients included in this study, reinforcing the hypothesis of MMPs involvement in the natural history of ID herniation. However, further studies are necessary to elucidate the exact role of MMPs in the resorption process of herniated lumbar discs. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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