The reduction of Na/H exchanger-3 protein and transcript expression in acute ischemia-reperfusion injury is mediated by extractable tissue factor(s).
Ischemic renal injury is a formidable clinical problem, the pathophysiology of which is incompletely understood. As the Na/H exchanger-3 (NHE3) mediates the bulk of apical sodium transport and a significant fraction of oxygen consumption in the proximal tubule, we examined mechanisms by which ischem...
| Publicado en: | Kidney International Vol. 80; no. 8; pp. 822 - 832 |
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| Autores principales: | , , , , , , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Elsevier B.V.
Oct2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104584652&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104584652 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00852538 4CU jtl: Kidney International issn: 00852538 maglogo: N pubinfo: dt: Oct2011 vid: 80 iid: 8 pid: 82545 pub: Elsevier B.V. place: Philadelphia, Pennsylvania artinfo: ui: 104584652 NLM21814178 2011298657 10.1038/ki.2011.229 NLM21814178 PMC4104484 104584652 ppf: 822 ppct: 10 formats: tig: atl: The reduction of Na/H exchanger-3 protein and transcript expression in acute ischemia-reperfusion injury is mediated by extractable tissue factor(s). aug: au: Di Sole F Hu MC Zhang J Babich V Bobulescu IA Shi M McLeroy P Rogers TE Moe OW Di Sole, Francesca Hu, Ming-Chang Zhang, Jianning Babich, Victor Bobulescu, I Alexandru Shi, Mingjun McLeroy, Paul Rogers, Thomas E Moe, Orson W Sole, Francesca Di Francesca Di, Sole affil: Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas 75390-8885, USA sug: subj: Reperfusion Injury Metabolism Biological Transport Physiology Thromboplastin Physiology Acute Disease Animals Cells Immunohistochemistry Mammals RNA Analysis Rats Biological Transport ab: Ischemic renal injury is a formidable clinical problem, the pathophysiology of which is incompletely understood. As the Na/H exchanger-3 (NHE3) mediates the bulk of apical sodium transport and a significant fraction of oxygen consumption in the proximal tubule, we examined mechanisms by which ischemia-reperfusion affects the expression of NHE3. Ischemia-reperfusion dramatically decreased NHE3 protein and mRNA (immunohistochemistry, immunoblot, and RNA blot) in rat kidney cortex and medulla. The decrease in NHE3 protein was uniform throughout all tubules, including those appearing morphologically intact. In the kidney cortex, a decrease in NHE3 surface protein preceded that of NHE3 total protein and mRNA. Kidney homogenates from rats exposed to mild renal ischemia-reduced cell surface NHE3 protein expression in opossum kidney cells in vitro, whereas homogenates from animals with moderate-to-severe ischemia reduced both total NHE3 protein and mRNA. The decrease in total NHE3 protein was dependent on the proteasomal degradation associated with NHE3 ubiquitylation measured by coimmunoprecipitation. The transferable factor(s) from the ischemic homogenate that reduce NHE3 expression were found to be heat sensitive and to be associated with a lipid-enriched fraction, and did not include regulatory RNAs. Thus, transferable factor(s) mediate the ischemia-reperfusion injury-induced decrease in NHE3 of the kidney. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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