| Sumario: | Objective: Neonatal hypoxic-ischemic (HI) insult has acute and long term deleterious effects on many tissues including the peripheral nerves. To date, no study has investigated the role of platelet-activating factor (PAF) antagonists on peripheral nerve damage in a neonatal rat model of HI. In this study, we examined the effects of PAF antagonist (ABT-491) on peripheral nerve damage in rats in the 16th week that were exposed to HI on 7th day after birth. Material and Methods: Seven-day-old Wistar rat pups were subjected to right common carotid artery ligation and hypoxia (92% nitrogen and 8% oxygen) for one hour. They were treated either with ABT-491 (n=19) or saline (n=20) immediately after hypoxia. In sham group (n=20), neither ligation nor hypoxia was performed. The compound motor action potential (CMAP) recordings of all animals were made in the sixteenth week following the HI. For CMAP recordings, bipolar stimulating electrodes were placed on the sciatic nerve. Upon stimulation, two surface electrodes, placed over the gastrocnemius muscle, recorded compound muscle action potentials. Results: The amplitude of CMAP recorded from the rats treated with saline after hypoxia was smaller compared to the sham group. However, the CMAP recorded from the group treated with ABT-491 after HI was not significantly different from the sham group. Conclusion: This study implies that HI has axonal damage on peripheral nerve but a PAF antagonist ABT-491 has a preventive effect on the axonal dysfunction after HI.
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