Determination of PER-1 and OXA-1 -like beta-lactamases in Ceftazidime-Resistant Pseudomonas aeruginosa Isolates by Molecular Methods.
Objective: Pseudomonas aeruginosa is one of the important nosocomial pathogens and resistant to many antibiotics including ?-lactams. PER-1 and OXA-10 type extended-spectrum ?-lactamases (ESBLs), the major ?-lactamases, were identified in P. aeruginosa. The aim of this study was to identify PER-1 (b...
| Publicado en: | Turkiye Klinikleri Journal of Medical Sciences Vol. 31; no. 5; pp. 1227 - 1236 |
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| Autores principales: | , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Turkiye Klinikleri
Oct2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104607257&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104607257 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13000292 1VOP jtl: Turkiye Klinikleri Journal of Medical Sciences issn: 13000292 maglogo: N pubinfo: dt: Oct2011 vid: 31 iid: 5 pid: 67298 pub: Turkiye Klinikleri artinfo: ui: 104607257 104607257 2011385615 10.5336/medsci.2010-22180 104607257 ppf: 1227 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Determination of PER-1 and OXA-1 -like beta-lactamases in Ceftazidime-Resistant Pseudomonas aeruginosa Isolates by Molecular Methods. aug: au: Ünlü Sögüt, Mehtap Yildirim, Tuba Birinci, Asuman Durupinar, Belma sug: subj: Antibiotics Adverse Effects Drug Resistance, Microbial Pseudomonas Infections Complications Cross Infection Electrophoresis Human Microbial Culture and Sensitivity Tests Phenotype Polymerase Chain Reaction ab: Objective: Pseudomonas aeruginosa is one of the important nosocomial pathogens and resistant to many antibiotics including ?-lactams. PER-1 and OXA-10 type extended-spectrum ?-lactamases (ESBLs), the major ?-lactamases, were identified in P. aeruginosa. The aim of this study was to identify PER-1 (blaPER-1) and OXA-10 (blaOXA-10)-like ?-lactamases in ceftazidime- resistant nosocomial P. aeruginosa strains. Material and Methods: The presence of PER-1 and OXA-10 like ?-lactamases was investigated by polymerase chain reaction in 50 ceftazidime-resistant P. aeruginosa strains isolated from patients hospitalized in various clinics of Ondokuz May?s University, School of Medicine between 2007 and 2008. The PER and OXA-10-like ?-lactamases were analyzed by restriction fragment length polymorphism (RFLP) and followed by pulsed-field gel electrophoresis (PFGE) for the determination of clonal relationship of the strains. Results: The blaPER-1 gene and blaOXA-10 like gene were detected in 23 (46%) and 39 (78%) of the 50 of ceftazidime-resistant P. aeruginosa isolates, respectively. In addition, both of the two ß-lactamase genes were also detected in 12 (23%) of the isolates. PER-1 and OXA-10, -11, -14, -16 types were identified by RFLP analysis. Although the PFGE typing results yielded 11 different banding patterns, 74% (n= 37) of the all P. aeruginosa strains were included in four main patterns. Conclusion: It was concluded that the prevalence of PER-1 and OXA-10 enzymes was common among ceftazidime resistant P. aeruginosa isolates. PER-1 and OXA-10 enzymes produced the isolates were being transmitted horizontally as the most of the isolates were clonally related. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: Turkish refInfo: holdings: @attributes: islocal: N |
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