Discrimination Analysis of Mass Spectrometry Proteomics for Lung Adenocarcinoma Detection.
Objective: To screen for the potential protein biomarkers in serum for the diagnosis of lung adenocarcinoma using proteomic fingerprint technology. Methods: Proteomic fingerprint technology combining magnetic beads (SED) with Matrix-assisted laser desorption/ionization time-of-flight mass spectromet...
| Publicado en: | Laboratory Medicine Vol. 42; no. 6; pp. 344 - 350 |
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| Autores principales: | , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Oxford University Press / USA
Jun2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104626532&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104626532 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00075027 4CY jtl: Laboratory Medicine issn: 00075027 maglogo: N pubinfo: dt: Jun2011 vid: 42 iid: 6 pid: 10398 pub: Oxford University Press / USA artinfo: ui: 104626532 70268042 10.1309/LMXWEJV3FFDR0DHH 104626532 ppf: 344 ppct: 6 formats: fmt: @attributes: type: P tig: atl: Discrimination Analysis of Mass Spectrometry Proteomics for Lung Adenocarcinoma Detection. aug: au: Jin Hong Dai Ze-ning Jin Chun-lan Hong Jin Ze-ning Dai Chun-lan Jin affil: Department of Laboratory, Sir Run Run Shaw Hospital of the Medicine School of Zhejiang University, Hangzhou; Key Laboratory of Biotherapy of Zhejiang Province, Hangzhou, People's Republic of China sug: subj: Lung Neoplasms Diagnosis Adenocarcinoma Diagnosis Proteomics Tumor Markers, Biological Blood Mass Spectrometry Methods Human Protein Array Analysis T-Tests Decision Trees Bioinformatics ab: Objective: To screen for the potential protein biomarkers in serum for the diagnosis of lung adenocarcinoma using proteomic fingerprint technology. Methods: Proteomic fingerprint technology combining magnetic beads (SED) with Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to profile and compare the serum proteins from 138 patients with lung adenocarcinoma and 120 healthy blood donors. Results: A total of 38 discriminating m/z peaks were identified that were related to lung adenocarcinoma (P<0.01). Four differently expressed potential biomarkers were identified with the relative molecular weights of 11644.1Da, 6298.7Da, 5840.4Da, and 3975.2Da, respectively. Among them 2 proteins with m/z 6298.7Da, 3975.2Da decreased, and 2 proteins with m/z 5840.4Da, 11644.1Da increased in lung adenocarcinoma. This diagnostic model can distinguish lung adenocarcinoma from healthy controls with a sensitivity of 92.85% and a specificity of 91.25%. Conclusions: Biomarkers for lung adenocarcinoma could be discovered in serum by MALDI-TOF-MS combining the use of SED. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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