A preliminary investigation of the impact of catechol-O-methyltransferase genotype on the absorption and metabolism of green tea catechins.

Purpose: Green tea is thought to possess many beneficial effects on human health. However, the extent of green tea polyphenol biotransformation may affect its proposed therapeutic effects. Catechol-O-methyltransferase (COMT), the enzyme responsible for polyphenolic methylation, has a common polymorp...

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Published in:European Journal of Nutrition Vol. 51; no. 1; pp. 47 - 56
Main Authors: Miller, Rosalind, Jackson, Kim, Dadd, Tony, Nicol, Beate, Dick, Joanne, Mayes, Andrew, Brown, A., Minihane, Anne
Format: research tables/charts Journal Article
Published: Springer Nature Feb2012
Online Access:View this record in EBSCOhost
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      dt: Feb2012
      vid: 51
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      pub: Springer Nature
      place: New York, New York
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        10.1007/s00394-011-0189-0
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        atl: A preliminary investigation of the impact of catechol-O-methyltransferase genotype on the absorption and metabolism of green tea catechins.
      aug:
        au:
          Miller, Rosalind
          Jackson, Kim
          Dadd, Tony
          Nicol, Beate
          Dick, Joanne
          Mayes, Andrew
          Brown, A.
          Minihane, Anne
        affil: Department of Food and Nutritional Sciences, University of Reading, Whiteknights Reading RG6 6AP UK
      sug:
        subj:
          Green Tea
          Plant Extracts Pharmacokinetics
          Genotype
          Polyphenols Pharmacokinetics
          United Kingdom
          Human
          Pilot Studies
          Prospective Studies
          Body Mass Index Evaluation
          Middle Age
          Descriptive Statistics
          Chromatography, High Pressure Liquid
          Polyphenols Blood
          Biological Availability
          Data Analysis Software
          Male
          Female
          Comparative Studies
          Middle Aged: 45-64 years
          Male
          Female
      ab: Purpose: Green tea is thought to possess many beneficial effects on human health. However, the extent of green tea polyphenol biotransformation may affect its proposed therapeutic effects. Catechol-O-methyltransferase (COMT), the enzyme responsible for polyphenolic methylation, has a common polymorphism in the genetic code at position 158 reported to result in a 40% reduction in enzyme activity in in vitro studies. The current preliminary study was designed to investigate the impact of COMT genotype on green tea catechin absorption and metabolism in humans. Methods: Twenty participants (10 of each homozygous COMT genotype) were recruited, and plasma concentration profiles were produced for epigallocatechin gallate (EGCG), epigallocatechin (EGC), epicatechin gallate (ECG), epicatechin (EC) and 4′-O-methyl EGCG after 1.1 g of Sunphenon decaffeinated green tea extract (836 mg green tea catechins), with a meal given after 60 min. Results: For the entire group, EGCG, EGC, EC, ECG and 4′-O-methyl EGCG reached maximum concentrations of 1.09, 0.41, 0.33, 0.16 and 0.08 μM at 81.5, 98.5, 99.0, 85.5 and 96.5 min, respectively. Bimodal curves were observed for the non-gallated green tea catechins EGC and EC as opposed to single-peaked curves for the gallated green tea catechins EGCG and ECG. No significant parametric differences between COMT genotype groups were found. Conclusions: In conclusion, the COMT Val(158/108)Met does not appear to have a dramatic influence on EGCG absorption and elimination. However, further pharmacokinetic research is needed to substantiate these findings.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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