The Effect of L-NAME Treatment on Leptin Associated Retinal Nitrosylation in Hypercarbic Oxygen Induced Retinopathy in Newborn Rats.
Objective: To investigate the biochemical effects of leptin and leptin plus L-NAME on retinal nitrotyrosine, malondialdehyde, and superoxide dismutase levels in a model of hypercarbic oxygen induced retinopathy in rats. Material and Methods: Forty newborn rat pups were divided into four groups consi...
| Publicado en: | Turkiye Klinikleri Journal of Medical Sciences Vol. 31; no. 4; pp. 780 - 785 |
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| Autores principales: | , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Turkiye Klinikleri
Aug2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104636540&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104636540 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13000292 1VOP jtl: Turkiye Klinikleri Journal of Medical Sciences issn: 13000292 maglogo: N pubinfo: dt: Aug2011 vid: 31 iid: 4 pid: 67298 pub: Turkiye Klinikleri artinfo: ui: 104636540 104636540 2011333655 10.5336/medsci.2010-19371 104636540 ppf: 780 ppct: 5 formats: fmt: @attributes: type: P tig: atl: The Effect of L-NAME Treatment on Leptin Associated Retinal Nitrosylation in Hypercarbic Oxygen Induced Retinopathy in Newborn Rats. aug: au: Özdemir, Gökhan Tolun, Fatma I Imrek, Seçil sug: subj: Malondialdehyde Therapeutic Use Retinopathy of Prematurity Drug Therapy Animal Studies Descriptive Statistics Infant, Newborn Kruskal-Wallis Test Mann-Whitney U Test Models, Biological Rats Spectrophotometers Superoxide Dismutase Infant, Newborn: birth-1 month ab: Objective: To investigate the biochemical effects of leptin and leptin plus L-NAME on retinal nitrotyrosine, malondialdehyde, and superoxide dismutase levels in a model of hypercarbic oxygen induced retinopathy in rats. Material and Methods: Forty newborn rat pups were divided into four groups consisting of leptin, leptin plus L-NAME, hyperoxia and sham groups. All groups, except the sham group, were exposed to ten minutes of hypercarbia followed by ten minutes of hyperoxia twice a day with an interval of 30 minutes. This protocol was performed for three days and then pups were sacrificed. The retinas were dissected and retinal nitrotyrosine, superoxide dismutase and malondialdehyde levels were measured. Results: The highest retinal nitrotyrosine levels were measured in the leptin group followed by the hyperoxia group (p< 0.05). Leptin plus L-NAME and the sham group showed similar results, indicating an antioxidative role for L-NAME (p< 0.05). Superoxide dismutase levels were also lower in the Leptin plus L-NAME group when compared to the hyperoxia group (p< 0.05). Conclusion: This study showed that leptin-induced retinal oxidation may be prevented by nitric oxide synthase inhibitors. We showed the protective role of L-NAME against nitric oxide mediated peroxynitrite toxicity. Further research should be carried out to investigate the role of nitrotyrosine on oxygen induced retinopathy. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: Turkish refInfo: holdings: @attributes: islocal: N |
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