Reduced Mitogenicity of Sera Following Weight Loss in Premenopausal Women.
We investigated whether serum from normal weight women is less mitogenic and more apoptotic than sera from the same women in the overweight state. Sera from premenopausal women, age (mean ± SEE) 34.6 ± 0.53 years, who were randomized to caloric restriction (CR) (n = 13), CR + aerobic exercise (AE) (...
| Publicado en: | Nutrition & Cancer Vol. 63; no. 6; pp. 916 - 924 |
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| Autores principales: | , , , , |
| Formato: | research tables/charts randomized controlled trial Journal Article |
| Publicado: |
Taylor & Francis Ltd
2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104674123&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104674123 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01635581 7MS jtl: Nutrition & Cancer issn: 01635581 maglogo: N pubinfo: dt: 2011 vid: 63 iid: 6 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 104674123 64320249 10.1080/01635581.2011.594209 NLM21774593 104674123 ppf: 916 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Reduced Mitogenicity of Sera Following Weight Loss in Premenopausal Women. aug: au: Azrad, Maria Chang, Pi-Ling Gower, Barbara A. Hunter, Gary R. Nagy, Tim R. affil: Department of Nutrition Sciences, University of Alabama at Birmingham, Birmingham, Alabama, USA sug: subj: Weight Loss Blood Premenopause Apoptosis Cells Metabolism Neoplasms Risk Factors Human Funding Source Female Adult Randomized Controlled Trials Exercise Diet United States Confidence Intervals Analysis of Variance Data Analysis Software Adult: 19-44 years Female ab: We investigated whether serum from normal weight women is less mitogenic and more apoptotic than sera from the same women in the overweight state. Sera from premenopausal women, age (mean ± SEE) 34.6 ± 0.53 years, who were randomized to caloric restriction (CR) (n = 13), CR + aerobic exercise (AE) (n = 14), or CR + resistance training (RT) (n = 20) were used to culture endometrial cancer cells. Phases of the cell cycle were determined, and proliferating cell nuclear antigen (PCNA) positivity was used to assess proliferation and apoptosis was assessed by determining cleaved caspase-3 and poly-ADP-ribose polymerase (PARP). Analyses showed that overall, cells grown in sera from the weight-reduced state had significantly more cells in G0/G1 and significantly fewer cells in the S and G2/M phases of the cell cycle than cells grown in sera from the overweight state. PCNA staining confirmed that cells grown in sera from the weight-reduced state had fewer proliferating cells. Cleaved caspase-3 and PARP were not different in cells grown in sera from the weight-reduced state compared to the overweight state. We conclude that weight loss with or without exercise could lower the risk for cancer through changes in serum that result in reduced cellular mitogenicity. pubtype: Academic Journal doctype: research tables/charts randomized controlled trial Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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