Methods for enumerating Escherichia coli in subtropical waters.
The standard membrane filtration method of the UK has been modified in order to improve its specificity for enumerating Escherichia coli in the subtropical waters of Hong Kong. This involves incorporating into the membrane lauryl sulphate (mLS) method either an in situ urease test (the mLS-UA method...
| Publicado en: | Epidemiology & Infection Vol. 106; no. 2; pp. 345 - 355 |
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| Autores principales: | , , , |
| Formato: | research Journal Article |
| Publicado: |
Cambridge University Press
Mar1991
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104749951&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104749951 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09502688 2S0 jtl: Epidemiology & Infection issn: 09502688 maglogo: N pubinfo: dt: Mar1991 vid: 106 iid: 2 pid: 15979 pub: Cambridge University Press artinfo: ui: 104749951 104749951 NLM2019302 2011108456 10.1017/S0950268800048494 NLM2019302 PMC2272005 104749951 ppf: 345 ppct: 10 formats: tig: atl: Methods for enumerating Escherichia coli in subtropical waters. aug: au: Cheung, W H Ha, D K Yeung, K Y Hung, R P affil: Environmental Protection Department, Southorn Centre, Wanchai, Hong Kong. sug: subj: Colony Count, Microbial Methods Escherichia Coli Water Microbiology False Positive Results Water Hong Kong Predictive Value of Tests ab: The standard membrane filtration method of the UK has been modified in order to improve its specificity for enumerating Escherichia coli in the subtropical waters of Hong Kong. This involves incorporating into the membrane lauryl sulphate (mLS) method either an in situ urease test (the mLS-UA method), or an in situ beta-glucuronidase test (the mLS-GUD method). The false-positive errors of the mLS-UA and mLS-GUD methods are low, ranging from 3-5%. A comparison between the membrane filtration (mLS-UA) method and the multiple tube technique in testing E. coli in subtropical beach-waters has demonstrated that the former can give much more precise counts, and is the method of choice for such a purpose. The mLS-GUD method, for which automated counting of E. coli colonies is possible, is a good alternative to mLS-UA in routine enumeration of this bacterial indicator in environmental waters. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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