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Molecular typing of Helicobacter pylori isolates from asymptomatic, ulcer and gastritis patients by urease gene polymorphism.

The gastric-adapted bacterium Helicobacter pylori plays an important role in gastritis and ulcer disease, but no phenotypic typing scheme presently exists for this organism. With a view to the development of genotypic typing, we have compared isolates of H. pylori from gastritis or ulcer patients wi...

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Published in:Epidemiology & Infection Vol. 112; no. 1; pp. 151 - 161
Main Authors: Desai, M, Linton, D, Owen, R J, Stanley, J
Format: research Journal Article
Published: Cambridge University Press Jan1994
Online Access:View this record in EBSCOhost
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      dt: Jan1994
      vid: 112
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      pub: Cambridge University Press
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        atl: Molecular typing of Helicobacter pylori isolates from asymptomatic, ulcer and gastritis patients by urease gene polymorphism.
      aug:
        au:
          Desai, M
          Linton, D
          Owen, R J
          Stanley, J
        affil: National Collection of Type Cultures, Central Public Health Laboratory, London.
      sug:
        subj:
          Gastritis Microbiology
          Helicobacter Infections Microbiology
          Helicobacter Pylori Classification
          Peptic Ulcer Microbiology
          Urease
          Bacterial Typing Techniques
          Nucleotides
          Blotting, Southern
          Cluster Analysis
          DNA Probes
          DNA Analysis
          DNA
          Genes
          Genetics
          Genotype
          Helicobacter Pylori
          Human
          Documentation
          Polymerase Chain Reaction
          Polymorphism, Genetic
      ab: The gastric-adapted bacterium Helicobacter pylori plays an important role in gastritis and ulcer disease, but no phenotypic typing scheme presently exists for this organism. With a view to the development of genotypic typing, we have compared isolates of H. pylori from gastritis or ulcer patients with those from subjects exhibiting no disease. Variation was analysed at the urease genes, ureA and ureCD, by employing PCR-generated probes in genomic Southern blot hybridizations. Whilst ureA restriction fragments provided a fourfold subgrouping of strains, ureCD fragments were considerably more discriminatory. Twenty-four combined ureACD profiles were generated with Hind III, subdividing the 64 strains into 11 types and 13 single profiles. The most prevalent profile (UI) was found in 33% of strains, almost all from gastritis or ulcer patients. On the other hand strains isolated from asymptomatic individuals had the most diverse ureACD profiles. A key finding from this set of isolates was that strains of H. pylori associated with general gastroduodenal disease were genetically more homogeneous than strains carried by people without disease symptoms.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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