Specific polymorphisms of IL-1ß genes (-511, +3953) and their probable effects on IL-1ß production in idiopatic hypogonadotropic hypogonadism.
Objective: In the present study, we aimed to determine a possible association of specific polymorphisms of IL-1? genes with enhanced production of IL-1? in patients with idiopathic hypogonadotropic hypogonadism (IHH). Material and Methods: Forty-five male IHH patients who had no familial relationshi...
| Published in: | Turkiye Klinikleri Journal of Medical Sciences Vol. 30; no. 6; pp. 1814 - 1820 |
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| Main Authors: | , , , , , , , , |
| Format: | research tables/charts Journal Article |
| Published: |
Turkiye Klinikleri
Dec2010
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104812758&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104812758 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13000292 1VOP jtl: Turkiye Klinikleri Journal of Medical Sciences issn: 13000292 maglogo: N pubinfo: dt: Dec2010 vid: 30 iid: 6 pid: 67298 pub: Turkiye Klinikleri artinfo: ui: 104812758 104812758 2010937899 10.5336/medsci.2009-14707 104812758 ppf: 1814 ppct: 6 formats: fmt: @attributes: type: P tig: atl: Specific polymorphisms of IL-1ß genes (-511, +3953) and their probable effects on IL-1ß production in idiopatic hypogonadotropic hypogonadism. aug: au: Musabak, Ugur Bolu, Erol Sagkan, Rahsan Ilikci Tunca, Yusuf Güran, Sefik Gül, Davut Sengül, Ali Oktenli, Cagatay Kutlu, Mustafa sug: subj: Hypogonadism Familial and Genetic Polymorphism, Genetic Case Control Studies Chi Square Test Confidence Intervals Data Analysis Software Descriptive Statistics Enzyme-Linked Immunosorbent Assay Follicle-Stimulating Hormone Blood Genes Human Interleukins Metabolism Kruskal-Wallis Test Luteinizing Hormone Blood Male Mann-Whitney U Test Odds Ratio Polymerase Chain Reaction Post Hoc Analysis Testosterone Blood Male ab: Objective: In the present study, we aimed to determine a possible association of specific polymorphisms of IL-1? genes with enhanced production of IL-1? in patients with idiopathic hypogonadotropic hypogonadism (IHH). Material and Methods: Forty-five male IHH patients who had no familial relationship with each other and 58 ethnically matched healthy male controls were enrolled in the study. Patients were diagnosed with IHH according to failure in spontaneus puberty and decreased serum testosterone concentration below normal range for adults. Serum follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels were low or in normal range in patients with IHH. In this study, we analyzed the gene polymorphisms of IL-1? at positions -511 and +3953 and serum IL-1? levels in patients with IHH and control subjects. For this purpose, we used polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and enzyme-linked immunosorbent assay (ELISA) methods. Results: The frequency of genotype 2/2 of IL-1?-511 gene was significantly higher in patients with IHH compared to healthy control subjects (24.4% vs 8.6%, respectively, p= 0.019). Although there was no significant difference in serum concentrations of IL-1? between total patients with IHH and healthy controls, the levels in the patients with the 1/2 genotype of IL-1?-511 and with the 2/2 genotype of IL-1?-511 were found significantly higher than the levels of patients with the 1/1 genotype of IL-1?-511. Conclusion: Our results indicate that IL-1?-511 gene polimorphism may be the cause of increased IL-1? production in patients with IHH. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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