The high rate of multiple-species candidemia detected with fluorescence in situ hybridization (FISH) methods: what are the clinical and prognostic implications?
Objective: The fluorescence in situ hybridization (FISH) with rRNA-targeted probes is a molecular method that allows identification of microorganisms in mixed assemblages from direct clinical samples without cultivitation. The aim of this study was to investigate the clinical features and risk facto...
| Publicado en: | Turkiye Klinikleri Journal of Medical Sciences Vol. 30; no. 6; pp. 1944 - 1953 |
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| Autores principales: | , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Turkiye Klinikleri
Dec2010
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104812790&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104812790 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13000292 1VOP jtl: Turkiye Klinikleri Journal of Medical Sciences issn: 13000292 maglogo: N pubinfo: dt: Dec2010 vid: 30 iid: 6 pid: 67298 pub: Turkiye Klinikleri artinfo: ui: 104812790 104812790 2010937931 10.5336/medsci.2009-14236 104812790 ppf: 1944 ppct: 9 formats: fmt: @attributes: type: P tig: atl: The high rate of multiple-species candidemia detected with fluorescence in situ hybridization (FISH) methods: what are the clinical and prognostic implications? aug: au: Börekci, Gülay Ersöz, Gülden Göksu, Musa Erdogan, Semra Emekdas, Gural affil: Mersin University Health School, Departments of Clinical Microbiology and Infectious Diseases, Biostatistic, Clinical Microbiology, Mersin University Faculty of Medicine, Mersin sug: subj: Candidiasis Diagnosis In Situ Hybridization, Fluorescence Academic Medical Centers Adult Aged Candidiasis Prognosis Candidiasis Symptoms Chi Square Test Data Analysis Software Descriptive Statistics Female Human Male Maximum Likelihood Microbial Culture and Sensitivity Tests Middle Age Polymerase Chain Reaction Turkiye Adult: 19-44 years Aged: 65+ years Middle Aged: 45-64 years Female Male ab: Objective: The fluorescence in situ hybridization (FISH) with rRNA-targeted probes is a molecular method that allows identification of microorganisms in mixed assemblages from direct clinical samples without cultivitation. The aim of this study was to investigate the clinical features and risk factors in patients with single and multiple-species candidemia detected with FISH method and also to evaluate the episodes of breakthrough candidemia. Material and Methods: A total of 325 positive blood culture samples were examined between January and August 2004 in medical microbiology laboratory of Mersin University Hospital. The demographic and clinical data of 45 patients with candidemia detected with conventional culture methods, FISH and PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) were analyzed retrospectively, single and multiple-species candidemia were investigated among patients with candidemia and these patients were compared with respect to risk factors. Results: Thirty five (77.8%) of 45 patients with candidemia had a single Candida species and 10 (22.2%) had multiple-species detected by FISH method. Of these 45 cases, 31.1% had associated solid and hematologic malignancies. The mean duration of hospitalization was 27 (2-180) days. All patients had at least one indwelling catheter (urinary catheter: 80%, peripheric venous catheter: 55.6%, central venous catheter: 26.7%). Most of the patients (95.6%) had been treated with broad spectrum antibiotics. The candidemias were mostly caused by non-albicans Candida spp., particularly Candida parapsilosis (71.1%). The rate of breakthrough candidemia was detected in 11.1% of the patients with candidemia and all of these were in patients with single species candidemia. Surprisingly, in case of single-species candidemia, the mortality rate (77.8%) was higher than that of multiple-species candidemia (22.2%). Conclusion: According to our data, the indwelling catheters and treatment with multiple antibiotics were considered as the risk factors for single and multiple-species candidemia. Although the rate of multiple-species candidemia detected with FISH method was found to be high, clinical and prognostic significance of multiple-species candidemia are still debatable. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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