Immunohistochemical analysis of MCC, TARC and CD 104 antigens in human lung tissue.
Objective: The aim of this study was to investigate the the distribution of CMA1 protein (MCC), CCL17 protein (TARC) and Integrin beta 4 (CD 104) monoclonal antibodies in normal adult human lung tissue. Material and Methods: In this study we examined the lung biopsy specimens obtained from the adult...
| Publicado en: | Turkiye Klinikleri Journal of Medical Sciences Vol. 30; no. 6; pp. 1971 - 1979 |
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| Autores principales: | , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Turkiye Klinikleri
Dec2010
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104812800&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104812800 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13000292 1VOP jtl: Turkiye Klinikleri Journal of Medical Sciences issn: 13000292 maglogo: N pubinfo: dt: Dec2010 vid: 30 iid: 6 pid: 67298 pub: Turkiye Klinikleri artinfo: ui: 104812800 2010937945 10.5336/medsci.2009-13920 104812800 ppf: 1971 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Immunohistochemical analysis of MCC, TARC and CD 104 antigens in human lung tissue. aug: au: Korkmaz, Metin Akkus, Murat Yildirim, Ayse Baran, Özlem Pamukcu Aktas, Ayfer affil: Department of Histology and Embriology, Bursa State Hospital, Bursa sug: subj: Antigens Immunohistochemistry Lung Physiology Proteins Academic Medical Centers Biopsy Human In Vitro Studies Turkiye ab: Objective: The aim of this study was to investigate the the distribution of CMA1 protein (MCC), CCL17 protein (TARC) and Integrin beta 4 (CD 104) monoclonal antibodies in normal adult human lung tissue. Material and Methods: In this study we examined the lung biopsy specimens obtained from the adult patients who underwent operations for different reasons in the Department of Lung Surgery at Dicle University Hospital, Diyarbak?r, Turkey. The tissue samples were immediately frozen in liquid nitrogen at -196º C. The samples were immunostained by indirect immune peroxidase technique. As primary antibodies, MCC, TARC and CD104 were used. As secondary antibodies, 1:200 rabbit anti - mouse IgG peroxidase diluted in PBS/BSA and 1:100 normal human serum solution were used. Negative control staining was performed using irrelevant mouse monoclonal antibodies omitting the primary antibody step. Sections were examined and photographed by Olympus BH2 light microscope. Results: We observed moderate reaction with vascular endothelial cells and with vascular smooth muscle cells with MCC which was aimed to show overall the presence of human lung mast cells. TARC, which was aimed to express the follicular dentritic cells, moderately reacted with follicular dentritic cells, bronchial smooth muscle cells and vascular endothelial cells. CD104, which is the integrin ?4 subunit that plays an important role in the adhesion of epithelium to basement membranes, moderately reacted with vascular endothelial cells and mainly reacted with the follicular dentritic cells of bronchi. Conclusion: In our study, it was defined that the expressions of these molecules have important role in understanding the function of human lung tissue. We reached the conclusion that our study, by presenting the difference between the normal lung tissue and pathologic lung tissue with expression the related proteins immunohistochemically may constitute preliminary findings for excluding the diseases like interstisial lung disease or chronic obstructive lung disease for the aim of. Our preliminary findings may shed light on future studies. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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