In Vitro Studies on the Inhibition of Colon Cancer by Butyrate and Polyphenolic Compounds.
Our aim was to investigate the effect of several dietary polyphenols on uptake of 14C-butyrate (14C-BT) by Caco-2 cells and try to correlate this effect with the modulation of the anticarcinogenic effect of BT in these cells. Acutely, uptake of 14C-BT (10 μM) was decreased by resveratrol, quercetin,...
| Publicado en: | Nutrition & Cancer Vol. 63; no. 2; pp. 282 - 295 |
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| Autores principales: | , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104820639&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104820639 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01635581 7MS jtl: Nutrition & Cancer issn: 01635581 maglogo: N pubinfo: dt: 2011 vid: 63 iid: 2 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 104820639 58529352 10.1080/01635581.2011.523166 NLM21207318 104820639 ppf: 282 ppct: 13 formats: fmt: @attributes: type: P tig: atl: In Vitro Studies on the Inhibition of Colon Cancer by Butyrate and Polyphenolic Compounds. aug: au: Goncalves, Pedro Araujo, Joao R. Pinho, M. Joao Martel, Fatima affil: Department of Biochemistry, Faculty of Medicine, University of Porto, Porto, Portugal sug: subj: Colonic Neoplasms Prevention and Control In Vitro Studies Butyric Acids Therapeutic Use Phenols Therapeutic Use Nutrition Colorectal Neoplasms Mortality United States Plants, Medicinal Antioxidants Therapeutic Use Fatty Acids Dietary Fiber Analysis Cell Culture Techniques Methods Gene Expression Profiling Polymerase Chain Reaction Methods DNA Physiology Oxidation-Reduction Cell Physiology Biological Assay Apoptosis Physiology Proteins Physiology Data Analysis Methods Analysis of Variance RNA Physiology ab: Our aim was to investigate the effect of several dietary polyphenols on uptake of 14C-butyrate (14C-BT) by Caco-2 cells and try to correlate this effect with the modulation of the anticarcinogenic effect of BT in these cells. Acutely, uptake of 14C-BT (10 μM) was decreased by resveratrol, quercetin, myricetin, and chrysin, and increased by xanthohumol, catechin, and epicatechin; and uptake of 14C-BT (20 mM) was reduced by resveratrol, quercetin, myricetin, chrysin, EGCG, and epicatechin. Resveratrol acts as a competitive inhibitor of 14C-BT uptake. Chronically, quercetin and EGCG increased uptake of 14C-BT (10 μM), whereas myricetin, rutin, chrysin, and xanthohumol decreased it. Moreover, catechin (1 μM), quercetin, myricetin, rutin, EGCG, and chrysin increased uptake of 14C-BT (20 mM), whereas catechin (0.1 μM) decreased it. EGCG, myricetin, and catechin decreased MCT1 mRNA expression, while chrysin increased it; quercetin, rutin, and xanthohumol had no effect. BT (5 mM; 48 h) markedly decreased cellular viability and proliferation and increased cell differentiation and apoptosis. In general, combination of polyphenolic compounds with BT did not significantly modify these changes. In conclusion, changes in uptake of BT induced by polyphenols do not correlate with changes on the effect of BT upon cell viability, cell proliferation, differentiation, and apoptosis. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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