Ethanol reduces zincosome formation in cultured astrocytes.
Aims: Zinc is an ion that participates in basic cellular and tissular functions. Zinc deficiency is present in many physiological and health problems affecting most body organs, including the brain. Among the circumstances involved in zinc deficiency, ethanol consumption is probably one of the most...
| Publicado en: | Alcohol & Alcoholism Vol. 46; no. 1; pp. 17 - 26 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial research Journal Article |
| Publicado: |
Oxford University Press / USA
Jan/Feb2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104825100&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104825100 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 07350414 FA3 jtl: Alcohol & Alcoholism issn: 07350414 maglogo: N pubinfo: dt: Jan/Feb2011 vid: 46 iid: 1 pid: 622 pub: Oxford University Press / USA artinfo: ui: 104825100 104825100 2010953188 NLM21123366 104825100 ppf: 17 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Ethanol reduces zincosome formation in cultured astrocytes. aug: au: Ballestín, Raúl Molowny, Asunción Marín, María Pilar Esteban-Pretel, Guillermo Romero, Ana María Lopez-Garcia, Carlos Renau-Piqueras, Jaime Ponsoda, Xavier affil: Biologia Cellular, Universitat de València, Avda. Dr. Moliner 50, 46100 Burjassot, Spain sug: subj: Alcoholism Complications Ethanol Pharmacodynamics Macrophages Classification Zinc Deficiency Animal Studies Equipment and Supplies Funding Source Microscopy, Electron Rats T-Tests Zinc Physiology ab: Aims: Zinc is an ion that participates in basic cellular and tissular functions. Zinc deficiency is present in many physiological and health problems affecting most body organs, including the brain. Among the circumstances involved in zinc deficiency, ethanol consumption is probably one of the most frequent. A dietary zinc supplement has been proposed as possibly being an efficient method to palliate zinc deficiency. Astrocytes form part of the hematoencephalic barrier, and they are apparently implicated in the homeostasis of the neuronal medium. In this work, we analyze the effect of ethanol on extracellular zinc management by rat astrocytes in culture. Methods: Intracellular levels of ‘free zinc ions’, in controls and 30 mM ethanol-treated astrocytes, were visualized by using the zinc fluorochrome TSQ. Cytoplasmic fluorescence and zincosome formation were measured after adding extracellular 50 µM ZnSO4 to cell monolayers. Zincosomes were also observed at the electron microscopy level. Results: Exposure to ethanol for 7 days lowered the basal zinc levels of astrocytes by ∼30%. This difference was consistently maintained after the zinc pulse. Zinc ions were confined to bright fluorescent particles, the ‘zincosomes’, which appeared to be formed by the endocytic pathway. Zincosomes were less abundant in alcohol-treated cells, indicating a deficit in endocytoses as the origin of low zinc intake in astrocytes after ethanol treatment. Conclusions: Ethanol reduces both intracellular ionic zinc levels and extracellular zinc uptake, resulting in poorer zincosome formation. Given the endocytic nature of zincosomes, the effect of ethanol on membrane trafficking is apparently the origin of this deficit. pubtype: Academic Journal doctype: pictorial research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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