Vasculogenic stem cell mobilization and wound recruitment in diabetic patients: Increased cell number and intracellular regulatory protein content associated with hyperbaric oxygen therapy.
Diabetic patients undergoing hyperbaric oxygen therapies (HBOT) for refractory lower extremity neuropathic ulcers exhibit more than a twofold elevation ( p=0.004) in circulating stem cells after treatments and the post-HBOT CD34 cell population contains two- to threefold higher levels of hypoxia ind...
| Publicado en: | Wound Repair & Regeneration Vol. 19; no. 2; pp. 149 - 162 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Mar/Apr2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104827809&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104827809 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Mar/Apr2011 vid: 19 iid: 2 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104827809 58725575 10.1111/j.1524-475X.2010.00660.x NLM21362081 104827809 ppf: 149 ppct: 13 formats: fmt: @attributes: type: P tig: atl: Vasculogenic stem cell mobilization and wound recruitment in diabetic patients: Increased cell number and intracellular regulatory protein content associated with hyperbaric oxygen therapy. aug: au: Thom, Stephen R. Milovanova, Tatyana N. Yang, Ming Bhopale, Veena M. Sorokina, Elena M. Uzun, Günalp Malay, D. Scot Troiano, Michael A. Hardy, Kevin R. Lambert, David S. Logue, Christopher J. Margolis, David J. affil: Institute for Environmental Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania,; Department of Emergency Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania, sug: subj: Diabetes Mellitus Physiopathology Wound Healing Physiology Proteins Analysis Hyperbaric Oxygenation Human Funding Source Diabetic Foot Therapy Stem Cells Physiology Biopsy Phlebotomy Digital Imaging Repeated Measures Analysis of Variance Descriptive Statistics ab: Diabetic patients undergoing hyperbaric oxygen therapies (HBOT) for refractory lower extremity neuropathic ulcers exhibit more than a twofold elevation ( p=0.004) in circulating stem cells after treatments and the post-HBOT CD34 cell population contains two- to threefold higher levels of hypoxia inducible factors-1, -2, and -3, as well as thioredoxin-1 ( p<0.003), than cells present in blood before HBOT. Skin margins obtained from 2-day-old abdominal wounds exhibit higher expression of CD133, CD34, hypoxia inducible factor-1, and Trx-1 vs. margins from refractory lower extremity wounds and expression of these proteins in all wounds is increased due to HBOT ( p<0.003). HBOT is known to mobilize bone marrow stem cells by stimulating nitric oxide synthase. We found that nitric oxide synthase activity is acutely increased in patients' platelets following HBOT and remains elevated for at least 20 hours. We conclude that HBOT stimulates vasculogenic stem cell mobilization from bone marrow of diabetics and more cells are recruited to skin wounds. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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