Polymerase I and transcript release factor regulates lipolysis via a phosphorylation-dependent mechanism.
Objective: Polymerase I and transcript release factor (PTRF) is a protein highly expressed in adipose tissue and is an integral structural component of caveolae. Here, we report on a novel role of PTRF in lipid mobilization.Research Design and Methods: PTRF expression was examined in different adipo...
| Publicado en: | Diabetes Vol. 60; no. 3; pp. 757 - 766 |
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| Autores principales: | , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
American Diabetes Association
Mar2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104834782&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104834782 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00121797 1G6 jtl: Diabetes issn: 00121797 maglogo: N pubinfo: dt: Mar2011 vid: 60 iid: 3 pid: 1367 pub: American Diabetes Association place: Arlington, Virginia artinfo: ui: 104834782 NLM21282370 2010967115 10.2337/db10-0744 NLM21282370 PMC3046836 104834782 ppf: 757 ppct: 9 formats: tig: atl: Polymerase I and transcript release factor regulates lipolysis via a phosphorylation-dependent mechanism. aug: au: Aboulaich N Chui PC Asara JM Flier JS Maratos-Flier E Aboulaich, Nabila Chui, Patricia C Asara, John M Flier, Jeffrey S Maratos-Flier, Eleftheria affil: Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA sug: subj: Adipocytes Metabolism Adipose Tissue Metabolism Lipid Metabolism, Inborn Errors Proteins Metabolism Adipocytes Adipocytes Drug Effects Adipose Tissue Drug Effects Adrenergic beta-Agonists Pharmacodynamics Animal Studies Blotting, Western Cells Chromatography, Liquid Immunity Insulin Pharmacodynamics Isoproterenol Pharmacodynamics Mass Spectrometry Metabolism Drug Effects Metabolism Physiology Mice Proteins Reverse Transcriptase Polymerase Chain Reaction RNA RNA Metabolism ab: Objective: Polymerase I and transcript release factor (PTRF) is a protein highly expressed in adipose tissue and is an integral structural component of caveolae. Here, we report on a novel role of PTRF in lipid mobilization.Research Design and Methods: PTRF expression was examined in different adipose depots of mice during fasting, refeeding, and after administration of catecholamines and insulin. Involvement of PTRF during lipolysis was studied upon PTRF knockdown and overexpression and mutation of PTRF phosphorylation sites in 3T3-L1 adipocytes.Results: PTRF expression in mouse white adipose tissue (WAT) is regulated by nutritional status, increasing during fasting and decreasing to baseline after refeeding. Expression of PTRF also is hormonally regulated because treatment of mice with insulin leads to a decrease in expression, whereas isoproterenol increases expression in WAT. Manipulation of PTRF levels revealed a role of PTRF in lipolysis. Lentiviral-mediated knockdown of PTRF resulted in a marked attenuation of glycerol release in response to isoproterenol. Conversely, overexpressing PTRF enhanced isoproterenol-stimulated glycerol release. Mass-spectrometric analysis revealed that PTRF is phosphorylated at multiple sites in WAT. Mutation of serine 42, threonine 304, or serine 368 to alanine reduced isoproterenol-stimulated glycerol release in 3T3-L1 adipocytes.Conclusions: Our study is the first direct demonstration for a novel adipose tissue-specific function of PTRF as a mediator of lipolysis and also shows that phosphorylation of PTRF is required for efficient fat mobilization. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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