Controlling fibroblast adhesion with pH modified polyelectrolyte multilayers.
Tissue cells need to adhere to a biomaterial surface to promote their growth and differentiation and, thus, foster the integration of implants. As a result, surface features and their modification play an important role in biomedical applications. In this study, the layer-by-layer (LbL) technique wa...
| Publicado en: | International Journal of Artificial Organs Vol. 34; no. 2; pp. 185 - 192 |
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| Autores principales: | , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Sage Publications Inc.
2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104841394&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104841394 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03913988 50IJ jtl: International Journal of Artificial Organs issn: 03913988 maglogo: Y pubinfo: dt: 2011 vid: 34 iid: 2 pid: 344 pub: Sage Publications Inc. place: Thousand Oaks, California artinfo: ui: 104841394 NLM21374559 2010974184 10.5301/IJAO.2011.6395 NLM21374559 104841394 ppf: 185 ppct: 7 formats: tig: atl: Controlling fibroblast adhesion with pH modified polyelectrolyte multilayers. aug: au: Niepel MS Peschel D Groth T Niepel, Marcus S Peschel, Dieter Groth, Thomas affil: Biomedical Materials Group, Department of Pharmaceutical Technology and Biopharmacy, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle, Saale, Germany sug: subj: Biocompatible Materials Cell Physiology Culture Media Fibroblasts Physiology Heparin Polyethylenes Tissue Engineering Methods Adsorption Cells Fibronectins Metabolism Human Hydrogen-Ion Concentration Microscopy Surface Properties ab: Tissue cells need to adhere to a biomaterial surface to promote their growth and differentiation and, thus, foster the integration of implants. As a result, surface features and their modification play an important role in biomedical applications. In this study, the layer-by-layer (LbL) technique was used to design self-assembled polyelectrolyte multilayer (PEM) coatings of polyethyleneimine (PEI) and heparin (HEP) on glass, which will control the adhesion of primary human dermal fibroblasts in a model system. The study showed that, among other surface features, the wettability of surfaces can be controlled by changing the conditions during multilayer self-assembly. Here, the pH value of the HEP solution was adjusted to acidic or alkaline values for terminal layers, which also led to a change in multilayer growth. Further, the study revealed that plain terminal layers were rather cytophobic. Upon pre-adsorption of fibronectin (FN), a clear effect on cell adhesion and morphology in dependence on the pH setup was evident. Proliferation studies clearly showed that terminal layers, which impaired cell adhesion, also inhibited growth of human fibroblasts under serum-conditions. On the other hand, on layers with pronounced cell adhesion an elevated cell growth was also observed. As a result, HEP terminated multilayers are interesting for applications requiring cell repellent properties, whereas PEI terminated multilayers could be used to promote cell adhesion and growth on implant surfaces. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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