Early cardiac gene transcript levels in peripheral blood mononuclear cells in patients with untreated essential hypertension.
Objectives: To assess the expression of early cardiac genes, implicated in the hypertrophic growth response of the adult heart, in peripheral blood mononuclear cells in patients with essential hypertension and its relationship to ambulatory blood pressure monitoring (ABPM) parameters and to echocard...
| Publicado en: | Journal of Hypertension Vol. 29; no. 4; pp. 791 - 798 |
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| Autores principales: | , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Lippincott Williams & Wilkins
2011 Apr
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104841465&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104841465 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 02636352 IQF jtl: Journal of Hypertension issn: 02636352 maglogo: N pubinfo: dt: 2011 Apr vid: 29 iid: 4 pid: 433 pub: Lippincott Williams & Wilkins place: Baltimore, Maryland artinfo: ui: 104841465 104841465 NLM21157370 2010974255 10.1097/HJH.0b013e3283424bc4 NLM21157370 104841465 ppf: 791 ppct: 7 formats: tig: atl: Early cardiac gene transcript levels in peripheral blood mononuclear cells in patients with untreated essential hypertension. aug: au: Kontaraki JE Marketou ME Zacharis EA Parthenakis FI Vardas PE Kontaraki, Joanna E Marketou, Maria E Zacharis, Evangelos A Parthenakis, Fragiskos I Vardas, Panos E affil: Molecular Cardiology Laboratory, School of Medicine, University of Crete, and Department of Cardiology, University Hospital of Heraklion, Crete, Greece sug: subj: Hypertension Metabolism Monocytes Metabolism RNA Metabolism Aged Female Hypertension Ultrasonography Male Middle Age Reverse Transcriptase Polymerase Chain Reaction Aged: 65+ years Middle Aged: 45-64 years Female Male ab: Objectives: To assess the expression of early cardiac genes, implicated in the hypertrophic growth response of the adult heart, in peripheral blood mononuclear cells in patients with essential hypertension and its relationship to ambulatory blood pressure monitoring (ABPM) parameters and to echocardiographic left ventricular mass. Methods: Twenty-four-hour ABPM, echocardiography and blood sampling were performed in 62 untreated participants with essential hypertension. Blood samples from 38 healthy individuals were included for comparison. Peripheral blood mononuclear cells (PBMCs) were isolated and gene transcript levels were determined by quantitative real-time reverse transcription PCR. Results: Myocardin (3.92±0.68 versus 2.09±0.67, P<0.001), GATA4 (3.48±0.68 versus 0.32±0.08, P<0.001) and Nkx2.5 (208.91±35.01 versus 129.75±49.70, P<0.001) were upregulated in hypertensive patients compared with controls. In hypertensive patients, transcript levels of myocardin (r=0.698, P<0.001) and GATA4 (r=0.374, P=0.003) showed significant positive correlations with 24-h systolic blood pressure (BP) as well as with mean BP, (r=0.626, P<0.001) and (r=0.340, P=0.007), respectively. A significant positive correlation between myocardin and 24-h pulse pressure (r=0.467, P<0.001) was also observed. Myocardin (r=-0.606, P<0.001) and GATA4 (r=-0.453, P<0.001) transcript levels also showed significant negative correlations with the mean 24-h dipping status. Additionally, myocardin (r=0.341, P=0.007), GATA4 (r=0.337, P=0.007) and Nkx2.5 (r=0.325, P=0.010) transcript levels showed significant positive correlations with left ventricular mass index. Conclusion: Myocardin and GATA4 transcript levels correlate significantly with 24-h ABPM parameters, rendering them potential candidate biomarkers in hypertension. Early cardiac gene transcript levels in PBMCs of hypertensive patients are associated with left ventricular mass and may reflect activation of the hypertrophic response gene network in these patients. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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