Effects of acute oral administration of vitamin C on the mouse liver transcriptome.
Vitamin C is a strong antioxidant that alters gene expression in cells, and its effects can be modified by cellular oxidative stress. We investigated the genome-wide effects of vitamin C on the in vivo transcriptome in the liver, which synthesizes various enzymes and proteins to defend against cellu...
| Published in: | Journal of Medicinal Food Vol. 14; no. 3; pp. 181 - 195 |
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| Main Authors: | , , , , , , |
| Format: | research tables/charts Journal Article |
| Published: |
Mary Ann Liebert, Inc.
2011 Mar
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104847411&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104847411 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1096620X C1S jtl: Journal of Medicinal Food issn: 1096620X maglogo: N pubinfo: dt: 2011 Mar vid: 14 iid: 3 pid: 1365 pub: Mary Ann Liebert, Inc. place: New Rochelle, New York artinfo: ui: 104847411 104847411 2010981452 10.1089/jmf.2010.1087 NLM21332396 104847411 ppf: 181 ppct: 14 formats: tig: atl: Effects of acute oral administration of vitamin C on the mouse liver transcriptome. aug: au: Jun, Hee-Jin Kim, Sukyung Dawson, Kevin Choi, Dal-Woong Kim, Jong-Sang Rodriguez, Raymond L. Lee, Sung-Joon affil: Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea sug: subj: Ascorbic Acid Pharmacodynamics Genes Drug Effects Liver Drug Effects Administration, Oral Animal Studies Antioxidants Biochips Blood Glucose Analysis Body Weights and Measures Cholesterol Blood Chronic Disease Prevention and Control Comparative Studies Data Analysis Software Descriptive Statistics Energy Metabolism Drug Effects Fasting Free Radicals Funding Source Histological Techniques In Vivo Studies Liver Analysis Mice Oxidative Stress Reverse Transcriptase Polymerase Chain Reaction RNA Analysis Signal Transduction South Korea Statistical Significance Triglycerides Blood ab: Vitamin C is a strong antioxidant that alters gene expression in cells, and its effects can be modified by cellular oxidative stress. We investigated the genome-wide effects of vitamin C on the in vivo transcriptome in the liver, which synthesizes various enzymes and proteins to defend against cellular oxidative stress. We fed mice vitamin C (0.056 mg/g of body weight) for 1 week and performed DNA microarray analysis with hepatic mRNA in fasting and refeeding states to mimic physiological conditions of oxidative stress. Significance analysis of microarray data identified approximately 6,000 genes differentially expressed in both fasting and refeeding states. In the fasting state, vitamin C induced overall energy metabolism as well as radical scavenging pathways. These were ameliorated in the refeeding state. These findings suggest that vitamin C has profound and immediate global effects on hepatic gene expression, which may help prevent oxidative stress, and that long-term treatment with vitamin C might reduce the risk of chronic disease. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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