Oral brush biopsy analysis by matrix assisted laser desorption/ionisation-time of flight mass spectrometry profiling - A pilot study.
Oral squamous cell carcinomas (OSCCs) often present as advanced tumours requiring aggressive local and regional therapy and result in significant functional impairment. The objective is to develop pre-symptomatic screening detection of OSCC by a brush biopsy method which is less invasive than the co...
| Publicado en: | Oral Oncology Vol. 47; no. 4; pp. 278 - 282 |
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| Autores principales: | , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Pergamon Press - An Imprint of Elsevier Science
Apr2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104854953&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104854953 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13688375 JRJ jtl: Oral Oncology issn: 13688375 maglogo: N pubinfo: dt: Apr2011 vid: 47 iid: 4 pid: 2410 pub: Pergamon Press - An Imprint of Elsevier Science artinfo: ui: 104854953 104854953 2010990884 10.1016/j.oraloncology.2011.02.005 NLM21354855 104854953 ppf: 278 ppct: 4 formats: tig: atl: Oral brush biopsy analysis by matrix assisted laser desorption/ionisation-time of flight mass spectrometry profiling - A pilot study. aug: au: Remmerbach TW Maurer K Janke S Schellenberger W Eschrich K Bertolini J Hofmann H Rupf S affil: Department of Oral & Maxillofacial and Facial Plastic Surgery, University of Leipzig, Leipzig, Germany; Griffith Health Institute, Gold Coast campus, Griffith University, QLD 4222, Australia. sug: subj: Carcinoma, Squamous Cell Pathology Early Detection of Cancer Methods Head and Neck Neoplasms Pathology Proteomics Methods Mass Spectrometry Methods Adult Aged Aged, 80 and Over Algorithms Biopsy Methods Female Gene Expression Profiling Methods Male Health Screening Middle Age Pilot Studies Proteomics Equipment and Supplies Sensitivity and Specificity Mass Spectrometry Equipment and Supplies Adult: 19-44 years Aged: 65+ years Aged, 80 & over Middle Aged: 45-64 years Female Male ab: Oral squamous cell carcinomas (OSCCs) often present as advanced tumours requiring aggressive local and regional therapy and result in significant functional impairment. The objective is to develop pre-symptomatic screening detection of OSCC by a brush biopsy method which is less invasive than the conventional biopsy for histology. Given the molecular heterogeneity of oral cancer, it is unlikely that even a panel of tumour markers would provide accurate diagnosis. Therefore, approaches such as the matrix-assisted-laser-desorption/ionisation-time-of-flight-mass -spectrometry (MALDI-TOF-MS) allow several biomarkers or peptide profile patterns to be simultaneously assessed. Brush biopsies from 27 patients with histology-proven OSCCs plus 40 biopsies from 10 healthy controls were collected. MALDI-TOF-MS profiling was performed and additional statistical analysis of the data was used to classify the disease status according to the biological behaviour of the lesion. For classification a support vector machine algorithm was trained using spectra of brush biopsy samples to distinguish healthy control patients from patients with histology-proven OSCC. MALDI-TOF-MS was able to distinguish between healthy patients and OSCC patients with a sensitivity of 100% and specificity of 93%. In summary, MALDI-TOF-MS in combination with sophisticated bioinformatic methods can distinguish OSCC patients from non-cancer controls with excellent sensitivity and specificity. Further improvement and validation of this approach is necessary to determine its feasibility to assist the pre-symptomatic detection of head and neck cancer screening in routine daily practice. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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