Cellular sources and inducers of cytokines present in acute wound fluid.
Acute wounds contain many biological active molecules, including several cytokines and growth factors. However, the cellular sources of each molecule, as well as the stimuli inducing them, are poorly characterized. We quantified the levels of 27 cytokines, chemokines, and growth factors in acute wou...
| Publicado en: | Wound Repair & Regeneration Vol. 19; no. 3; pp. 337 - 348 |
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| Autores principales: | , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
May/Jun2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104887968&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104887968 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: May/Jun2011 vid: 19 iid: 3 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104887968 60368816 10.1111/j.1524-475X.2011.00668.x NLM21371163 104887968 ppf: 337 ppct: 11 formats: fmt: @attributes: type: P tig: atl: Cellular sources and inducers of cytokines present in acute wound fluid. aug: au: Grimstad, Øystein Sandanger, Øystein Ryan, Liv Otterdal, Kari Damaas, Jan Kristian Pukstad, Brita Espevik, Terje affil: Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway; Department of Dermatology, St Olavs Hospital HF, University Hospital, Trondheim, Norway sug: subj: Wounds and Injuries Physiopathology Fluids and Secretions Cytokines Human Funding Source Tissue Culture Techniques Immunoassay Reverse Transcriptase Polymerase Chain Reaction ab: Acute wounds contain many biological active molecules, including several cytokines and growth factors. However, the cellular sources of each molecule, as well as the stimuli inducing them, are poorly characterized. We quantified the levels of 27 cytokines, chemokines, and growth factors in acute wound fluid in a luminex-based assay. The acute wound fluid contained particularly high levels of IL-6 and IL-8, as well as elevated levels of MCP-1, IL-1RA, PDGF, IP-10, IFN-γ, and TNF-α. Surprisingly, the amounts of IL-1β and IL-10 were relatively low. To characterize the cellular sources of these molecules, we analyzed supernatants from monocytes, neutrophils, keratinocytes, fibroblasts, and endothelial cells stimulated with pro- and anti inflammatory cytokines, and different Toll-like receptor (TLR) ligands. The different cell types showed overlapping but distinct patterns of production of signal molecules, as well as sensitivity to ligands. Among pro-inflammatory cytokines, IL-1β was the most potent inducer of signal molecule production. Furthermore, keratinocytes and endothelial cells were in particular responsive to the Toll-like receptor-3 ligand polyI:C. New interactions between cytokines and growth factors were revealed, which may have important roles in wound healing, including IL-1β-induced IFN-γ and IL-10-induced VEGF. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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