Prevalence of Listeria monocytogenes in raw meats marketed in Bangkok and characterization of the isolates by phenotypic and molecular methods.
Listeria monocytogenes causes listeriosis characterized by septicaemia, encephalitis, and abortion or stillbirth. Regular monitoring of its prevalence in food and characterization of its phenotypes and genotypes are necessary for disease surveillance and tracing the epidemic outbreaks. In this study...
| Publicado en: | Journal of Health, Population & Nutrition Vol. 29; no. 1; pp. 26 - 39 |
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| Autores principales: | , , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
BioMed Central
Feb2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104890084&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104890084 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 16060997 HII jtl: Journal of Health, Population & Nutrition issn: 16060997 maglogo: N pubinfo: dt: Feb2011 vid: 29 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 104890084 NLM21528788 2011039461 10.3329/jhpn.v29i1.7565 NLM21528788 PMC3075055 104890084 ppf: 26 ppct: 13 formats: fmt: @attributes: type: P tig: atl: Prevalence of Listeria monocytogenes in raw meats marketed in Bangkok and characterization of the isolates by phenotypic and molecular methods. aug: au: Indrawattana N Nibaddhasobon T Sookrung N Chongsa-Nguan M Tungtrongchitr A Makino S Tungyong W Chaicumpa W Indrawattana, Nitaya Nibaddhasobon, Tanaporn Sookrung, Nitat Chongsa-Nguan, Manas Tungtrongchitr, Anchalee Makino, Sou-Ichi Tungyong, Witawat Chaicumpa, Wanpen affil: Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand sug: subj: Food Microbiology Methods Food Microbiology Statistics and Numerical Data Listeria Meat Microbiology Antibiotics Pharmacodynamics Genotype Listeria Classification Listeria Drug Effects Microbial Culture and Sensitivity Tests Methods Documentation Nucleotides Phenotype Polymerase Chain Reaction Methods Serotyping Methods Thailand Virulence ab: Listeria monocytogenes causes listeriosis characterized by septicaemia, encephalitis, and abortion or stillbirth. Regular monitoring of its prevalence in food and characterization of its phenotypes and genotypes are necessary for disease surveillance and tracing the epidemic outbreaks. In this study, the prevalence of L. monocytogenes in raw meats marketed in Bangkok was 15.4%. The bacteria isolated from meat were serotyped and genotyped using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). Their virulence-associated genes, antimicrobial susceptibility, and ability to invade intestinal epithelial cells were studied. All 22 L. monocytogenes strains isolated from 104 raw meat samples carried virulence-associated genes, such as actA, flaA, hlyA, lap, inlA, inlB, and prfA. These were serotype 4b, suggesting their pathogenic and epidemic potential. These isolates could be classified into six ERIC-PCR groups: A-E The majority (59.1%) of the isolates belonged to Group A, and three isolates were Group D which was closely related to the Group A. Two isolates each were Group C and E, and one isolate each was group B and F. Although the isolates belonged to the same serotype and genotype and were all equipped with the virulence-associated genes, they showed a different cell invasion capability and antibiotic susceptibility. All the isolates were susceptible to ampicillin, amikacin, chloramphenicol, gentamicin, imipenem, penicillin G, sulphamethoxazole-trimethoprim, and tetracycline. However, one isolate showed only intermediate susceptibility to tetracycline. The data provide the first molecular insight into the L. monocytogenes isolates in Thailand and elucidate a potential risk of people contracting listeriosis. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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