Interaction of oral bacteria with gingival epithelial cell multilayers.
Primary gingival epithelial cells were cultured in multilayers as a model for the study of interactions with oral bacteria associated with health and periodontal disease. Multilayers maintained at an air-liquid interface in low-calcium medium displayed differentiation and cytokeratin properties char...
| Publicado en: | Molecular Oral Microbiology Vol. 26; no. 3; pp. 210 - 221 |
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| Autores principales: | , , , , , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Wiley-Blackwell
Jun2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104891425&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104891425 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 20411006 B64Q jtl: Molecular Oral Microbiology issn: 20411006 maglogo: Y pubinfo: dt: Jun2011 vid: 26 iid: 3 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104891425 NLM21545698 2011041045 10.1111/j.2041-1014.2011.00609.x NLM21545698 PMC3248246 104891425 ppf: 210 ppct: 11 formats: tig: atl: Interaction of oral bacteria with gingival epithelial cell multilayers. aug: au: Dickinson BC Moffatt CE Hagerty D Whitmore SE Brown TA Graves DT Lamont RJ Dickinson, B C Moffatt, C E Hagerty, D Whitmore, S E Brown, T A Graves, D T Lamont, R J affil: Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL, USA sug: subj: Bacteria Periodontium Microbiology Gingiva Microbiology Mouth Mucosa Microbiology Gram-Negative Bacteria Immunology Gram-Negative Bacteria Physiology Apoptosis Physiology Bacteria Immunology Cell Culture Techniques Periodontium Periodontium Immunology Epithelial Cells Immunology Epithelial Cells Microbiology Gingiva Gingiva Immunology Image Processing, Computer Assisted Inflammation Mediators Analysis Interleukin 1 Analysis Interleukins Analysis Proteins Analysis Microscopy Periodontal Diseases Microbiology Gram-Negative Anaerobic Bacteria Immunology Gram-Negative Anaerobic Bacteria Physiology Streptococcus Immunology Streptococcus Physiology Time Factors Tumor Necrosis Factor Analysis ab: Primary gingival epithelial cells were cultured in multilayers as a model for the study of interactions with oral bacteria associated with health and periodontal disease. Multilayers maintained at an air-liquid interface in low-calcium medium displayed differentiation and cytokeratin properties characteristic of junctional epithelium. Multilayers were infected with fluorescently labeled Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum or Streptococcus gordonii, and bacterial association was determined by confocal microscopy and quantitative image analysis. Porphyromonas gingivalis invaded intracellularly and spread from cell to cell; A. actinomycetemcomitans and F. nucleatum remained extracellular and showed intercellular movement through the multilayer; whereas S. gordonii remained extracellular and predominantly associated with the superficial cell layer. None of the bacterial species disrupted barrier function as measured by transepithelial electrical resistance. P. gingivalis did not elicit secretion of proinflammatory cytokines. However, A. actinomycetemcomitans and S. gordonii induced interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), IL-6 and IL-8 secretion; and F. nucleatum stimulated production of IL-1β and TNF-α. Aggregatibacter actinomycetemcomitans, F. nucleatum and S. gordonii, but not P. gingivalis, increased levels of apoptosis after 24 h infection. The results indicate that the organisms with pathogenic potential were able to traverse the epithelium, whereas the commensal bacteria did not. In addition, distinct host responses characterized the interaction between the junctional epithelium and oral bacteria. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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