Discrimination of parotid neoplasms from the normal parotid gland by use of Raman spectroscopy and support vector machine.

Preoperative diagnosis of neoplasms in the parotid gland is essential for successful surgical treatment. The purpose of this study is to apply Raman spectroscopy in order to distinguish the spectral differences between pleomorphic adenoma and Warthin tumor from that of normal parotid gland tissues....

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Publicado en:Oral Oncology Vol. 47; no. 5; pp. 430 - 436
Autores principales: Yan B, Li Y, Yang G, Wen ZN, Li ML, Li LJ
Formato: research Journal Article
Publicado: Pergamon Press - An Imprint of Elsevier Science May2011
Acceso en línea:Ver este registro en EBSCOhost
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      dt: May2011
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      pub: Pergamon Press - An Imprint of Elsevier Science
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        104904875
        2011063595
        10.1016/j.oraloncology.2011.02.021
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        atl: Discrimination of parotid neoplasms from the normal parotid gland by use of Raman spectroscopy and support vector machine.
      aug:
        au:
          Yan B
          Li Y
          Yang G
          Wen ZN
          Li ML
          Li LJ
        affil: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
      sug:
        subj:
          Neoplasms, Complex and Mixed Diagnosis
          Adenoma Diagnosis
          Parotid Gland
          Parotid Neoplasms Diagnosis
          Spectrum Analysis, Raman Methods
          Neoplasms, Complex and Mixed Pathology
          Adenoma Pathology
          Adolescence
          Adult
          Aged
          Algorithms
          Diagnosis, Differential
          Female
          Male
          Middle Age
          Parotid Neoplasms Pathology
          Sensitivity and Specificity
          Young Adult
          Adolescent: 13-18 years
          Adult: 19-44 years
          Aged: 65+ years
          Middle Aged: 45-64 years
          Female
          Male
      ab: Preoperative diagnosis of neoplasms in the parotid gland is essential for successful surgical treatment. The purpose of this study is to apply Raman spectroscopy in order to distinguish the spectral differences between pleomorphic adenoma and Warthin tumor from that of normal parotid gland tissues. Furthermore we establish the diagnostic model of the Raman spectra of neoplasms in parotid gland by employing support vector machine (SVM) with Gaussian radial basis function. Firstly, Raman spectra from different histopathological tissues were obtained by near-infrared Raman microscope, SVM was then employed to analyze the different spectra and establish a discriminating model. As a result, the differences of peaks in the region 800-1800cm(-1) demonstrated the biochemical molecular alterations between different histopathological tissues. Compared with normal parotid gland tissues, the content of proteins, lipids and DNA increased in pleomorphic adenoma. The content of DNA increased but proteins and lipids decreased in Warthin tumor. SVM displayed a powerful role in the classification of three different groups. The sensitivities and specificities of discrimination between different groups reached above 95% and 99%, respectively. Raman spectroscopy combined SVM algorithm could have great potential for providing a noninvasive, effective and accurate diagnostic technology for neoplasm diagnosis in the parotid gland.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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