Electropermeabilization of endocytotic vesicles in B16 F1 mouse melanoma cells.
It has been reported previously that electric pulses of sufficiently high voltage and short duration can permeabilize the membranes of various organelles inside living cells. In this article, we describe electropermeabilization of endocytotic vesicles in B16 F1 mouse melanoma cells. The cells were e...
| Publicado en: | Medical & Biological Engineering & Computing Vol. 48; no. 5; pp. 407 - 414 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
May2010
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104909761&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104909761 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01400118 PO0 jtl: Medical & Biological Engineering & Computing issn: 01400118 maglogo: N pubinfo: dt: May2010 vid: 48 iid: 5 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104909761 NLM20361267 2010630163 10.1007/s11517-010-0599-9 NLM20361267 PMC2855809 104909761 ppf: 407 ppct: 7 formats: fmt: @attributes: type: P tig: atl: Electropermeabilization of endocytotic vesicles in B16 F1 mouse melanoma cells. aug: au: Batista Napotnik T Rebersek M Kotnik T Lebrasseur E Cabodevila G Miklavcic D Napotnik, Tina Batista Rebersek, Matej Kotnik, Tadej Lebrasseur, Eric Cabodevila, Gonzalo Miklavcic, Damijan affil: Faculty of Electrical Engineering, University of Ljubljana, Trzaska 25, 1000, Ljubljana, Slovenia sug: subj: Cytological Techniques Methods Cytoplasm Metabolism Melanoma Metabolism Animal Studies Cell Membrane Metabolism Cell Physiology Cells Heterocyclic Compounds Pharmacokinetics Isoquinolines Pharmacokinetics Mice ab: It has been reported previously that electric pulses of sufficiently high voltage and short duration can permeabilize the membranes of various organelles inside living cells. In this article, we describe electropermeabilization of endocytotic vesicles in B16 F1 mouse melanoma cells. The cells were exposed to short, high-voltage electric pulses (from 1 to 20 pulses, 60 ns, 50 kV/cm, repetition frequency 1 kHz). We observed that 10 and 20 such pulses induced permeabilization of membranes of endocytotic vesicles, detected by release of lucifer yellow from the vesicles into the cytosol. Simultaneously, we detected uptake of propidium iodide through plasma membrane in the same cells. With higher number of pulses permeabilization of the membranes of endocytotic vesicles by pulses of given parameters is accompanied by permeabilization of plasma membrane. However, with lower number of pulses only permeabilization of the plasma membrane was detected. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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