Static magnetic field exposure promotes differentiation of osteoblastic cells grown on the surface of a poly-L-lactide substrate.
This study investigated the effects of static magnetic fields on the differentiation of MG63 cells cultured on the surface of poly-L-lactide (PLLA) substrates. The cells were continuously exposed to a 4,000 Gauss-static magnetic field (SMF) for 5 days. The proliferation effects of the SMF were measu...
| Publicado en: | Medical & Biological Engineering & Computing Vol. 48; no. 8; pp. 793 - 799 |
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| Autores principales: | , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
Aug2010
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104912428&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104912428 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01400118 PO0 jtl: Medical & Biological Engineering & Computing issn: 01400118 maglogo: N pubinfo: dt: Aug2010 vid: 48 iid: 8 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 104912428 104912428 NLM20521172 2010713743 10.1007/s11517-010-0639-5 NLM20521172 104912428 ppf: 793 ppct: 6 formats: fmt: @attributes: type: P tig: atl: Static magnetic field exposure promotes differentiation of osteoblastic cells grown on the surface of a poly-L-lactide substrate. aug: au: Feng SW Lo YJ Chang WJ Lin CT Lee SY Abiko Y Huang HM Feng, Sheng-Wei Lo, Yi-June Chang, Wei-Jen Lin, Che-Tong Lee, Sheng-Yang Abiko, Yoshimitsu Huang, Haw-Ming affil: School of Dentistry, Taipei Medical University, Taipei, Taiwan, ROC sug: subj: Electromagnetic Fields Connective Tissue Cells Polyesters Culture Media Cell Differentiation Radiation Effects Cells Microscopy, Electron, Scanning Tissue Engineering Methods ab: This study investigated the effects of static magnetic fields on the differentiation of MG63 cells cultured on the surface of poly-L-lactide (PLLA) substrates. The cells were continuously exposed to a 4,000 Gauss-static magnetic field (SMF) for 5 days. The proliferation effects of the SMF were measured by MTT assay. Morphologic changes and extracellular matrix release were observed by scanning electron microscopy. The effects of the SMF on alkaline phosphatase activity levels were compared between exposed and unexposed cells. The SMF-exposed cells exhibited decreased MTT values after 1 and 3 days of culture. In addition, SMF exposure promoted the expression of extracellular matrix in MG63 cells on the PLLA substrate. After 1 day, the alkaline phosphatase-specific activity of SMF-exposed MG63 cells was significantly increased (P < 0.05) with a ratio of 1.5-fold. These results show that MG63 cells, seeded on a PLLA disc and treated with SMF, had a more differentiated phenotype. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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