Microsatellite typing and susceptibilities of serial Cryptococcus neoformans isolates from Cuban patients with recurrent cryptococcal meningitis.
Background: Cryptococcus neoformans is commonly associated with meningoencephalitis in immunocompromised patients and occasionally in apparently healthy individuals. Recurrence of infection after initial treatment is not uncommon. We studied C. neoformans isolates from 7 Cuban patients with recurren...
| Publicado en: | BMC Infectious Diseases Vol. 10; no. 1; pp. 289 - 290 |
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| Autores principales: | , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
BioMed Central
2010
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104931208&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104931208 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14712334 1CHU jtl: BMC Infectious Diseases issn: 14712334 maglogo: N pubinfo: dt: 2010 vid: 10 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 104931208 104931208 NLM20920321 2010831204 10.1186/1471-2334-10-289 NLM20920321 PMC2959061 104931208 ppf: 289 ppct: 1 formats: tig: atl: Microsatellite typing and susceptibilities of serial Cryptococcus neoformans isolates from Cuban patients with recurrent cryptococcal meningitis. aug: au: Illnait-Zaragozí MT Martínez-Machín GF Fernández-Andreu CM Hagen F Boekhout T Klaassen CH Meis JF Illnait-Zaragozí, María T Martínez-Machín, Gerardo F Fernández-Andreu, Carlos M Hagen, Ferry Boekhout, Teun Klaassen, Corné H W Meis, Jacques F affil: Department of Bacteriology and Mycology, Instituto Pedro Kourí, Havana, Cuba sug: subj: Antifungal Agents Pharmacodynamics Cryptococcus Classification Cryptococcus Drug Effects Meningitis, Cryptococcal Microbiology DNA Mycological Typing Techniques Blood Microbiology Cerebrospinal Fluid Microbiology Cryptococcus Cuba Female Genotype Male Microbial Culture and Sensitivity Tests Epidemiology, Molecular Recurrence Semen Microbiology Urine Microbiology Female Male ab: Background: Cryptococcus neoformans is commonly associated with meningoencephalitis in immunocompromised patients and occasionally in apparently healthy individuals. Recurrence of infection after initial treatment is not uncommon. We studied C. neoformans isolates from 7 Cuban patients with recurrent cryptococcal meningitis. Antifungal susceptibility and genotyping with microsatellite molecular typing were carried out.Methods: Isolates (n = 19) were recovered from cerebrospinal fluid, blood, urine and semen. Antifungal susceptibilities for amphotericin B, fluconazole, flucytosine, itraconazole, voriconazole, posaconazole and isavuconazole were tested by CLSI M27A3 broth microdilution method. Genotyping was done using a panel of 9 microsatellite (STR) markers: (CT)n, (TG)n, (TA)n, (CTA)n, (TCT)n, (CCA)n, (TTAT)n, (ATCC)n and (TATT)n.Results: The average number of isolates/patient was 2.71. The mean time interval between the collection of any two isolates was 52.5 days. All strains were identified as C. neoformans var. grubii (serotype Aα). Although none of the strains were resistant to the studied drugs, in serial isolates from two patients, MICs values of triazoles increased 4-5 log2 dilutions over time. STR patterns showed 14 distinctive profiles. In three patients the recurrent infection was associated with genotypically identical isolates. The four other patients had relapse isolates which were genotypically different from the initial infecting strain.Conclusion: Recurrences of cryptococcal meningitis in our series of patients was not associated with development of drug resistance of the original strain but by an initial infection with different strains or a reinfection with a new strain. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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