Intermittent hypoxia mobilizes bone marrow-derived very small embryonic-like stem cells and activates developmental transcriptional programs in mice.
BACKGROUND: Obstructive sleep apnea is a prevalent disorder associated with cognitive dysfunction and cardiovascular and metabolic morbidity and is characterized by recurrent episodes of hypoxia during sleep. Bone marrow-derived very small embryonic-like (VSEL) pluripotent stem cells represent a rec...
| Publicado en: | Sleep Vol. 33; no. 11; pp. 1439 - 1447 |
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| Autores principales: | , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Oxford University Press / USA
2010 Nov 1
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104952658&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104952658 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01618105 2EI jtl: Sleep issn: 01618105 maglogo: N pubinfo: dt: 2010 Nov 1 vid: 33 iid: 11 pid: 622 pub: Oxford University Press / USA artinfo: ui: 104952658 104952658 2010867266 NLM21102985 PMC2954693 104952658 ppf: 1439 ppct: 8 formats: tig: atl: Intermittent hypoxia mobilizes bone marrow-derived very small embryonic-like stem cells and activates developmental transcriptional programs in mice. aug: au: Gharib SA Dayyat EA Khalyfa A Kim J Clair HB Kucia M Gozal D sug: subj: Anoxia Blood Bone Marrow Metabolism Molecular Structure Stem Cells Metabolism Animal Studies Anoxia Physiopathology Biological Markers Blood Chemokines Blood Cytokines Blood Factor Analysis Flow Cytometry Methods Genes Hematopoietic Stem Cells Metabolism Male Mice Microarray Analysis Methods Models, Biological Male ab: BACKGROUND: Obstructive sleep apnea is a prevalent disorder associated with cognitive dysfunction and cardiovascular and metabolic morbidity and is characterized by recurrent episodes of hypoxia during sleep. Bone marrow-derived very small embryonic-like (VSEL) pluripotent stem cells represent a recruitable pool that may play an important role in organ repair after injury. We hypothesized that exposure to intermittent hypoxia (IH) can mobilize VSELs from the bone marrow (BM) to peripheral blood (PB) in mice and can activate distinct transcriptional programs. METHODS: Adult mice were exposed to IH or normoxia for 48 hours. VSELs were sorted from BM and PB using flow cytometry. Plasma levels of stem cell chemokines, stromal cell derived factor-1 (SDF-1), hepatocyte growth factor (HGF), and leukemia inhibitory factor (LIF) were measured. Transcriptional profiling of VSELs was performed, and differentially expressed genes were mapped to enriched functional categories and genetic networks. RESULTS: Exposure to IH elicited migration of VSELs from BM to PB and elevations in plasma levels of chemokines. More than 1100 unique genes were differentially expressed in VSELs in response to IH. Gene Ontology and network analysis revealed the activation of organ-specific developmental programs among these genes. CONCLUSIONS: Exposure to IH mobilizes VSELs from the BM to PB and activates distinct transcriptional programs in VSELs that are enriched in developmental pathways, including central nervous system development and angiogenesis. Thus, VSELs may serve as a reserve mobile pool of pluripotent stem cells that can be recruited into PB and may play an important role in promoting end-organ repair during IH. CITATION: Gharib SA; Dayyat EA; Khalyfa A; Kim J; Clair HB; Kucia M; Gozal D. Intermittent hypoxia mobilizes bone marrow-derived very small embryonic-like stem cells and activates developmental transcriptional programs in mice. SLEEP 2010;33(11):1439-1446. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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