Enhanced classification of Chagas serologic results and epidemiologic characteristics of seropositive donors at three large blood centers in Brazil.
Background: A major problem in Chagas disease donor screening is the high frequency of samples with inconclusive results. The objective of this study was to describe patterns of serologic results among donors to the three Brazilian REDS-II blood centers and correlate with epidemiologic characteristi...
| Publicado en: | Transfusion Vol. 50; no. 12 Part 1; pp. 2628 - 2638 |
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| Autores principales: | , , , , , , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Wiley-Blackwell
Dec2010
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104961560&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104961560 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00411132 1YB jtl: Transfusion issn: 00411132 maglogo: Y pubinfo: dt: Dec2010 vid: 50 iid: 12 Part 1 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 104961560 NLM20576017 2010874319 10.1111/j.1537-2995.2010.02756.x NLM20576017 PMC2997114 104961560 ppf: 2628 ppct: 10 formats: tig: atl: Enhanced classification of Chagas serologic results and epidemiologic characteristics of seropositive donors at three large blood centers in Brazil. aug: au: Sabino EC Salles NA Sarr M Barreto AM Oikawa M Oliveira CD Leao SC Carneiro-Proietti AB Custer B Busch MP Sabino, Ester C Salles, Nanci A Sarr, Moussa Barreto, Angela M Oikawa, Marcio Oliveira, Cláudia D Leao, Silvana C Carneiro-Proietti, Anna B Custer, Brian Busch, Michael P affil: Pró-Sangue Foundation and the University of São Paulo, São Paulo, Brazil sug: subj: Blood Banks Statistics and Numerical Data Blood Donors Classification Blood Donors Statistics and Numerical Data Trypanosomiasis Diagnosis Trypanosomiasis Epidemiology Adult Algorithms Blood Banks Methods Blood Banks Standards Brazil Trypanosomiasis Blood Trypanosomiasis Immunology Transplant Donors Female Human Male Epidemiological Research Serologic Tests Classification Serologic Tests Methods Serologic Tests Standards Protozoa Immunology Young Adult Adult: 19-44 years Female Male ab: Background: A major problem in Chagas disease donor screening is the high frequency of samples with inconclusive results. The objective of this study was to describe patterns of serologic results among donors to the three Brazilian REDS-II blood centers and correlate with epidemiologic characteristics.Study Design and Methods: The centers screened donor samples with one Trypanosoma cruzi lysate enzyme immunoassay (EIA). EIA-reactive samples were tested with a second lysate EIA, a recombinant-antigen based EIA, and an immunfluorescence assay. Based on the serologic results, samples were classified as confirmed positive (CP), probable positive (PP), possible other parasitic infection (POPI), and false positive (FP).Results: In 2007 to 2008, a total of 877 of 615,433 donations were discarded due to Chagas assay reactivity. The prevalences (95% confidence intervals [CIs]) among first-time donors for CP, PP, POPI, and FP patterns were 114 (99-129), 26 (19-34), 10 (5-14), and 96 (82-110) per 100,000 donations, respectively. CP and PP had similar patterns of prevalence when analyzed by age, sex, education, and location, suggesting that PP cases represent true T. cruzi infections; in contrast the demographics of donors with POPI were distinct and likely unrelated to Chagas disease. No CP cases were detected among 218,514 repeat donors followed for a total of 718,187 person-years.Conclusion: We have proposed a classification algorithm that may have practical importance for donor counseling and epidemiologic analyses of T. cruzi-seroreactive donors. The absence of incident T. cruzi infections is reassuring with respect to risk of window phase infections within Brazil and travel-related infections in nonendemic countries such as the United States. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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