Inhibition of murine skin carcinogenesis by freeze-dried grape powder and other grape-derived major antioxidants.

Overexposure of the skin to carcinogenic insults causes a variety of adverse effects, among them the development of skin carcinomas. Since there is a need to develop efficient chemopreventive agents based on nutrition, our goal was to determine antioxidant and anti-carcinogenic properties of grapes...

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Publicado en:Nutrition & Cancer Vol. 63; no. 1; pp. 28 - 39
Autores principales: Hanausek M, Spears E, Walaszek Z, Kowalczyk MC, Kowalczyk P, Wendel C, Slaga TJ
Formato: pictorial research tables/charts Journal Article
Publicado: Taylor & Francis Ltd 2011
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 2011
      vid: 63
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      pub: Taylor & Francis Ltd
      place: Philadelphia, Pennsylvania
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        10.1080/01635581.2010.516474
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        atl: Inhibition of murine skin carcinogenesis by freeze-dried grape powder and other grape-derived major antioxidants.
      aug:
        au:
          Hanausek M
          Spears E
          Walaszek Z
          Kowalczyk MC
          Kowalczyk P
          Wendel C
          Slaga TJ
        affil: The University of Texas Health Science Center at San Antonio, Department of Pharmacology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA
      sug:
        subj:
          Skin Neoplasms Prevention and Control
          Fruit
          Antioxidants
          Animal Studies
          Mice
          Models, Biological
          Funding Source
          Fisher's Exact Test
          Chi Square Test
          Data Analysis Software
          One-Way Analysis of Variance
      ab: Overexposure of the skin to carcinogenic insults causes a variety of adverse effects, among them the development of skin carcinomas. Since there is a need to develop efficient chemopreventive agents based on nutrition, our goal was to determine antioxidant and anti-carcinogenic properties of grapes by evaluating grape powder developed by the California Table Grape Commission. In order to elucidate the mechanism(s) of action of grape powder, three of the major antioxidant components found in grapes-resveratrol, catechin, quercetin, and grape seed extract, containing a proanthocyanidin B-2-gallate-were evaluated for their abilities to inhibit oxidative stress and to protect the immune system. Tested antioxidants given topically and/or systemically strongly inhibited 7,12-dimethylbenz[a]anthracene (DMBA)-induced epidermal hyperplasia, proliferation, and inflammation. The hydroxylation of 2'-deoxyguanosine was markedly inhibited by topical and dietary administration of test variables, i.e., by approximately 40-70%. Simultaneous dietary and topical treatment with antioxidants reduced these biomarkers, showing strong additive and in some combinations synergistic effects. DMBA-mediated Ha-ras mutations in codon 61 were reduced by up to 50% with topical applications, but much higher inhibition was observed in mice treated with different combinations. The results of the present study clearly show impressive effects of combined topical and dietary treatments with above grape-derived antioxidants.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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