Could alkali production be considered an approach for caries control?
This study investigated the relationship of arginine deiminase (ADS) and urease activities with dental caries through a case-control study. ADS and urease activities were measured in dental smooth-surface supragingival plaque and whole saliva samples from 93 subjects, who were in three different gro...
| Publicado en: | Caries Research Vol. 44; no. 6; pp. 547 - 555 |
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| Autores principales: | , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Karger AG
2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=104994831&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 104994831 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00086568 DEU jtl: Caries Research issn: 00086568 maglogo: N pubinfo: dt: 2011 vid: 44 iid: 6 pid: 2485 pub: Karger AG artinfo: ui: 104994831 NLM21071940 2010917603 10.1159/000321139 NLM21071940 PMC3202947 104994831 ppf: 547 ppct: 8 formats: tig: atl: Could alkali production be considered an approach for caries control? aug: au: Gordan, V V Garvan, C W Ottenga, M E Schulte, R Harris, P A McEdward, D Magnusson, I affil: Department of Operative Dentistry, University of Florida, Gainesville, Fla., USA. sug: subj: Dental Caries Dental Plaque Hydrolases Metabolism Streptococcus Mutans Urease Metabolism Adolescence Adult Alkalies Metabolism Alkalies Therapeutic Use Ammonia Metabolism Ammonia Therapeutic Use Bacteria Bacteria Metabolism Ecosystem Dental Caries Microbiology Dental Caries Prevention and Control Dental Caries Activity Tests Dental Plaque Microbiology Human Hydrogen-Ion Concentration Middle Age Saliva Saliva Microbiology Young Adult Adolescent: 13-18 years Adult: 19-44 years Middle Aged: 45-64 years ab: This study investigated the relationship of arginine deiminase (ADS) and urease activities with dental caries through a case-control study. ADS and urease activities were measured in dental smooth-surface supragingival plaque and whole saliva samples from 93 subjects, who were in three different groups: caries-free (n = 31), caries-active (n = 30), and caries-experienced (n = 32). ADS activity was measured by quantification of the ammonia generated from the incubation of plaque and saliva samples in a mixture containing 50 mM arginine-HCl and 50 mM Tris-maleate buffer, pH 6.0. ADS-specific activity was defined as nanomoles of ammonia generated per minute per milligram of protein. Urease activity was determined by quantification of ammonia produced from 50 mM urea. For bacterial identification and enumeration real-time qPCR analysis was used. Groups were compared using Kruskal-Wallis tests. Spearman correlations were used to analyze plaque metabolic activity and bacterial relationships. The results revealed significantly higher ammonia production from arginine in saliva (1.06 vs. 0.18; p < 0.0001) and plaque samples (1.74 vs. 0.58; p < 0.0001) from caries-free subjects compared to caries-active subjects. Urease levels were about 3-fold higher in the plaque of caries-free subjects (p < 0.0001). Although higher urease activity in saliva of caries-experienced and caries-free subjects was evident, no significant difference was found between the groups. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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