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Detection of airborne viruses in a pediatrics department measured using real-time qPCR coupled to an air-sampling filter method...quantitative polymerase chain reaction

Children are vulnerable to viral infections. The study discussed in this article investigates the possibility of aerosol transmission of viruses in children under age 18 in the pediatrics department of a medical center in Taipei, Taiwan. After first using the filtration method to collect viral aeros...

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Published in:Journal of Environmental Health Vol. 73; no. 4; pp. 22 - 29
Main Authors: Tseng C, Chang L, Li C
Format: research tables/charts Journal Article
Published: National Environmental Health Association Nov2010
Online Access:View this record in EBSCOhost
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      dt: Nov2010
      vid: 73
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      pub: National Environmental Health Association
      place: Denver, Colorado
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        atl: Detection of airborne viruses in a pediatrics department measured using real-time qPCR coupled to an air-sampling filter method...quantitative polymerase chain reaction
      aug:
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          Tseng C
          Chang L
          Li C
        affil: Assistant Professor, Department and Graduate Institute of Public Health, Tzu Chi University, Hualien, Taiwan, R.O.C. Room H409, 4F, 701 Sec. 3, Jhongyang Rd., Hualien 970, Taiwan
      sug:
        subj:
          Air Pollution, Indoor Evaluation
          Ambulatory Care Facilities Evaluation
          Emergency Service Evaluation
          Pediatric Care
          Virus Diseases Transmission
          Viruses Analysis
          Adolescence
          Chi Square Test
          Child
          Child, Preschool
          Descriptive Statistics
          Funding Source
          Human
          Infant
          Kruskal-Wallis Test
          P-Value
          Polymerase Chain Reaction
          Spearman's Rank Correlation Coefficient
          Taiwan
          Adolescent: 13-18 years
          Child: 6-12 years
          Child, Preschool: 2-5 years
          Infant: 1-23 months
      ab: Children are vulnerable to viral infections. The study discussed in this article investigates the possibility of aerosol transmission of viruses in children under age 18 in the pediatrics department of a medical center in Taipei, Taiwan. After first using the filtration method to collect viral aerosols, the authors combined it with real-time quantitative polymerase chain reaction (qPCR) to detect influenza A virus (INFAV), human adenovirns (HAdV), and enterovirus. Of 33 aerosol samples collected from the emergency room of the pediatrics department, 8 (24%) were positive for INFAV, 12 (36%) for HAdV, and 5 (15%) for enterovirus. HAdV was detected from the aerosol of 26 samples, but INFAV and enteroviruses were not. The filter and real-time qPCR can be used to detect and quantify the viral load in aerosols, in which enteroviruses had the highest viral titer. In summary, a well-established filter/real-time qPCR assay was successfully applied to measure viral aerosols in the occupational environment. Environmental monitoring of airborne viruses could provide an early indicator of dangerous viruses in the air of hospitals.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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