Effects of CYP2C19, MDR1, and interleukin 1-B gene variants on the eradication rate of Helicobacter pylori infection by triple therapy with pantoprazole, amoxicillin, and metronidazole.

Eradication of H. pylori is an important treatment strategy in peptic ulcer patients. Current regimens of eradication consist of proton pump inhibitor (PPI) and two antibiotics. Effects of PPI may depend on their metabolism, and other factors important for the pathophysiology of peptic ulcer disease...

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Publicado en:European Journal of Clinical Pharmacology Vol. 66; no. 7; pp. 681 - 688
Autores principales: Gawronska-Szklarz B, Siuda A, Kurzawski M, Bielicki D, Marlicz W, Drozdzik M
Formato: research tables/charts Journal Article
Publicado: Springer Nature Jul2010
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Jul2010
      vid: 66
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      pub: Springer Nature
      place: New York, New York
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        10.1007/s00228-010-0818-1
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        atl: Effects of CYP2C19, MDR1, and interleukin 1-B gene variants on the eradication rate of Helicobacter pylori infection by triple therapy with pantoprazole, amoxicillin, and metronidazole.
      aug:
        au:
          Gawronska-Szklarz B
          Siuda A
          Kurzawski M
          Bielicki D
          Marlicz W
          Drozdzik M
        affil: Department of Pharmacology, Pomeranian Medical University, Powstancow Wlkp. 72 70-111 Szczecin Poland
      sug:
        subj:
          Amoxicillin Administration and Dosage
          Genes
          Glycoproteins Physiology
          Helicobacter Infections Therapy
          Interleukins Physiology
          Metronidazole Administration and Dosage
          Pantoprazole Sodium Administration and Dosage
          Amoxicillin Blood
          Amoxicillin Therapeutic Use
          Descriptive Statistics
          Disease Management Methods
          Genotype
          Human
          Metronidazole Blood
          Metronidazole Therapeutic Use
          Pantoprazole Sodium Blood
          Pantoprazole Sodium Therapeutic Use
          Patient Care Methods
          Peptic Ulcer Risk Factors
          Peptic Ulcer Therapy
          Pharmacy and Pharmacology
          Polymorphism, Genetic
          Proteins Physiology
      ab: Eradication of H. pylori is an important treatment strategy in peptic ulcer patients. Current regimens of eradication consist of proton pump inhibitor (PPI) and two antibiotics. Effects of PPI may depend on their metabolism, and other factors important for the pathophysiology of peptic ulcer disease. Aim of the present study was to evaluate an association of CYP2C19, MDR1, and IL-1B polymorphisms with the eradication rate of H. pylori in Polish Caucasian patients treated with a triple therapy of pantoprazole, amoxicillin, and metronidazole. A total of 139 peptic ulcer patients, positive for H. pylori infection, were treated with triple therapy (pantoprazole + amoxicillin + metronidazole). Subsequently, the patients were divided into two groups (group 1, success, and group 2, failure of eradication after therapy) and genotyped by the PCR-RFLP method for the presence of CYP2C19 variant alleles (*2, *3, and *17), and MDR1 3435C>T and IL-1B +3954C>T polymorphisms. Pantoprazole serum concentrations were measured using the HPLC method. No significant differences in frequency or distribution of CYP2C19 genotypes were found between the two groups of patients (i.e., with successful H. pylori eradication and treatment failure). However, any carrier of defective CYP2C19*2/*2 genotype was found among patients with treatment failure. Similarly, MDR1 and IL-1B genotypes were found to be significantly associated with the success or failure of H. pylori eradication. Univariate and multivariate analysis of the genotypes did not reveal any significant association between the genotypes and H. pylori eradication. Pantoprazole concentrations differed significantly, and were the highest in patients with defective allele CYP2C19*2 carriers and lowest in hyperactive genotype homozygotes CYP2C19*17/*17. The results suggest that the CYP2C19 genotype contrary to MDR1 and IL-1B genotypes may have an impact on the efficacy of H. pylori eradication in peptic ulcer patients treated with pantoprazole in Polish Caucasian peptic ulcer patients administered pantoprazole, amoxicillin, and metronidazole.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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