Arsenic inhibits myogenic differentiation and muscle regeneration.
Background: The incidence of low birth weights is increased in offspring of women who are exposed to high concentrations of arsenic in drinking water compared with other women. We hypothesized that effects of arsenic on birth weight may be related to effects on myogenic differentiation. Objective: W...
| Publicado en: | Environmental Health Perspectives Vol. 118; no. 7; pp. 949 - 957 |
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| Autores principales: | , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
National Institute of Environmental Health Sciences
Jul2010
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105068592&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105068592 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00916765 3B5 jtl: Environmental Health Perspectives issn: 00916765 maglogo: N pubinfo: dt: Jul2010 vid: 118 iid: 7 pid: 56539 pub: National Institute of Environmental Health Sciences place: Research Triangle Park, North Carolina artinfo: ui: 105068592 2010731803 10.1289/ehp.0901525 NLM20299303 PMC2920914 105068592 ppf: 949 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Arsenic inhibits myogenic differentiation and muscle regeneration. aug: au: Yen Y Tsai K Chen Y Huang C Yang R Liu S affil: Institute of Toxicology College of Medicine, National Taiwan University, Taipei, Taiwan sug: subj: Arsenic Adverse Effects Cell Physiology Regeneration Analysis of Variance Biopsy Culture Media Descriptive Statistics Female Funding Source Human In Vitro Studies In Vivo Studies Mice Middle Age Muscle Fibers Analysis Staining and Labeling T-Tests Taiwan Middle Aged: 45-64 years Female ab: Background: The incidence of low birth weights is increased in offspring of women who are exposed to high concentrations of arsenic in drinking water compared with other women. We hypothesized that effects of arsenic on birth weight may be related to effects on myogenic differentiation. Objective: We investigated the effects of arsenic trioxide (As(2)O(3)) on the myogenic differentiation of myoblasts in vitro and muscle regeneration in vivo. Methods: C2C12 myoblasts and primary mouse and human myoblasts were cultured in differentiation media with or without As(2)O(3) (0.1-0.5 µM) for 4 days. Myogenic differentiation was assessed by myogenin and myosin heavy chain expression and multinucleated myotube formation in vitro; skeletal muscle regeneration was tested using an in vivo mouse model with experimental glycerol myopathy. Results: A submicromolar concentration of As(2)O(3) dose-dependently inhibited myogenic differentiation without apparent effects on cell viability. As(2)O(3) significantly and dose-dependently decreased phosphorylation of Akt and p70s6k proteins during myogenic differentiation. As(2)O(3)-induced inhibition in myotube formation and muscle-specific protein expression was reversed by transfection with the constitutively active form of Akt. Sections of soleus muscles stained with hematoxylin and eosin showed typical changes of injury and regeneration after local glycerol injection in mice. Regeneration of glycerol-injured soleus muscles, myogenin expression, and Akt phosphorylation were suppressed in muscles isolated from As(2)O(3)-treated mice compared with untreated mice. Conclusion: Our results suggest that As2O3 inhibits myogenic differentiation by inhibiting Aktregulated signaling. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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