Two-dimensional liquid chromatography technique coupled with mass spectrometry analysis to compare the proteomic response to cadmium stress in plants.
Plants are useful in studies of metal toxicity, because their physiological responses to different metals are correlated with the metal exposure dose and chemical state. Moreover a network of proteins and biochemical cascades that may lead to a controlled homeostasis of metals has been identified in...
| Publicado en: | Journal of Biomedicine & Biotechnology pp. 567510 - 567511 |
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| Autores principales: | , , |
| Formato: | research Journal Article |
| Publicado: |
Wiley-Blackwell
2010
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105082013&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105082013 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11107243 137K jtl: Journal of Biomedicine & Biotechnology issn: 11107243 maglogo: N pubinfo: dt: 2010 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 105082013 2010748392 10.1155/2010/567510 NLM20204056 PMC2828102 105082013 ppf: 567510 ppct: 1 formats: fmt: @attributes: type: P tig: atl: Two-dimensional liquid chromatography technique coupled with mass spectrometry analysis to compare the proteomic response to cadmium stress in plants. aug: au: Visioli G Marmiroli M Marmiroli N affil: Division of Genetics and Environmental Biotechnologies, Department of Environmental Sciences, University of Parma, Viale G.P. Usberti 11/A, 43100 Parma, Italy. sug: subj: Cadmium Chromatography, Liquid Methods Plants Drug Effects Proteomics Mass Spectrometry Methods Stress, Physiological Drug Effects Proteins Proteins Metabolism Plant Leaves Plant Leaves Drug Effects Plant Proteins Plant Proteins Metabolism Plants Plants Metabolism Reproducibility of Results ab: Plants are useful in studies of metal toxicity, because their physiological responses to different metals are correlated with the metal exposure dose and chemical state. Moreover a network of proteins and biochemical cascades that may lead to a controlled homeostasis of metals has been identified in many plant species. This paper focuses on the global protein variations that occur in a Populus nigra spp. clone (Poli) that has an exceptional tolerance to the presence of cadmium. Protein separation was based on a two-dimensional liquid chromatography technique. A subset of 20 out of 126 peaks were identified as being regulated differently under cadmium stress and were fingerprinted by MALDI-TOF. Proteins that were more abundant in the treated samples were located in the chloroplast and in the mitochondrion, suggesting the importance of these organelles in the response and adaptation to metal stress. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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