Size of AT(n) insertions in promoter region modulates Gmhsp17.6-L mRNA transcript levels.
During earlier experiments, an SSR molecular marker (176 Soy HSP) showing high correlation (70%) with resistance/susceptibility to javanese root-knot nematode Meloidogyne javanica was identified in soybean. After being sequenced, results indicated that the SSR 176 Soy HSP marker was inserted in the...
| Publicado en: | Journal of Biomedicine & Biotechnology pp. 847673 - 847674 |
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| Autores principales: | , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Wiley-Blackwell
2010
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105082066&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105082066 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11107243 137K jtl: Journal of Biomedicine & Biotechnology issn: 11107243 maglogo: N pubinfo: dt: 2010 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 105082066 2010748428 NLM20300590 PMC2837908 105082066 ppf: 847673 ppct: 1 formats: fmt: @attributes: type: P tig: atl: Size of AT(n) insertions in promoter region modulates Gmhsp17.6-L mRNA transcript levels. aug: au: Fuganti R Machado Mde F Lopes VS Silva JF Arias CA Marin SR Binneck E Abdelnoor RV Marcelino FC Nepomuceno AL affil: Genetics and Plant Breeding, Maringá State University (UEM), Colombo Avenue 5790, Paraná Maringá 87020-900, Brazil. sug: subj: DNA Proteins Plant Diseases Genes RNA Soybeans ab: During earlier experiments, an SSR molecular marker (176 Soy HSP) showing high correlation (70%) with resistance/susceptibility to javanese root-knot nematode Meloidogyne javanica was identified in soybean. After being sequenced, results indicated that the SSR 176 Soy HSP marker was inserted in the promoter region of Gmhsp17.6-L gene. It was also detected in this region that resistant genotypes presented insertions between AT(31) and AT(33) in size and susceptible genotypes, AT(9). Gmhsp17.6-L gene coding region presented a perfect match in amino acid sequence in all soybean genotypes. A ribonuclease protection assay showed that Gmhsp17.6-L gene mRNA transcripts were present in all genotypes. A real-time relative quantification (qPCR) indicated in the resistant individuals higher mRNA transcripts levels, which presented in the sequencing more AT(n) insertions. These results suggest that the number of AT(n) insertions inside this promoter region could modulate up or down gene levels. Those findings can lead to the possibility of manipulating, between some limits, the mRNA transcripts levels using different sizes of AT(n) insertions. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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