Microarray detection call methodology as a means to identify and compare transcripts expressed within syncytial cells from soybean (Glycine max) roots undergoing resistant and susceptible reactions to the soybean cyst nematode (Heterodera glycines)
Background. A comparative microarray investigation was done using detection call methodology (DCM) and differential expression analyses. The goal was to identify genes found in specific cell populations that were eliminated by differential expression analysis due to the nature of differential expres...
| Publicado en: | Journal of Biomedicine & Biotechnology pp. 30p - 31 |
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| Autores principales: | , , , , |
| Formato: | diagnostic images pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
2010
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105082208&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105082208 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11107243 137K jtl: Journal of Biomedicine & Biotechnology issn: 11107243 maglogo: N pubinfo: dt: 2010 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 105082208 105082208 2010748564 10.1155/2010/491217 NLM20508855 PMC2875038 105082208 ppf: 30p ppct: 1 formats: fmt: @attributes: type: P tig: atl: Microarray detection call methodology as a means to identify and compare transcripts expressed within syncytial cells from soybean (Glycine max) roots undergoing resistant and susceptible reactions to the soybean cyst nematode (Heterodera glycines) aug: au: Klink VP Overall CC Alkharouf NW MacDonald MH Matthews BF affil: Department of Biological Sciences, Harned Hall, Mississippi State University, Mississippi State, MS 39762, USA. sug: subj: Genes Nematodes Plants, Medicinal Soybeans Animal Studies Gene Expression Lasers Utilization ab: Background. A comparative microarray investigation was done using detection call methodology (DCM) and differential expression analyses. The goal was to identify genes found in specific cell populations that were eliminated by differential expression analysis due to the nature of differential expression methods. Laser capture microdissection (LCM) was used to isolate nearly homogeneous populations of plant root cells. Results. The analyses identified the presence of 13,291 transcripts between the 4 different sample types. The transcripts filtered down into a total of 6,267 that were detected as being present in one or more sample types. A comparative analysis of DCM and differential expression methods showed a group of genes that were not differentially expressed, but were expressed at detectable amounts within specific cell types. Conclusion. The DCM has identified patterns of gene expression not shown by differential expression analyses. DCM has identified genes that are possibly cell-type specific and/or involved in important aspects of plant nematode interactions during the resistance response, revealing the uniqueness of a particular cell population at a particular point during its differentiation process. pubtype: Academic Journal doctype: diagnostic images pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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