Optimal fluxes, reaction replaceability, and response to enzymopathies in the human red blood cell.

Characterizing the capabilities, key dependencies, and response to perturbations of genome-scale metabolic networks is a basic problem with important applications. A key question concerns the identification of the potentially most harmful reaction knockouts. The integration of combinatorial methods...

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Publicado en:Journal of Biomedicine & Biotechnology pp. 10p - 11
Autores principales: De Martino A, Granata D, Marinari E, Martelli C, Van Kerrebroeck V
Formato: equations & formulas research tables/charts Journal Article
Publicado: Wiley-Blackwell 2010
Acceso en línea:Ver este registro en EBSCOhost
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        atl: Optimal fluxes, reaction replaceability, and response to enzymopathies in the human red blood cell.
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          De Martino A
          Granata D
          Marinari E
          Martelli C
          Van Kerrebroeck V
        affil: CNR Institute for Physico-Chemical Processes (IPCF), Rome Sapienza Unit, Roma, Italy.
      sug:
        subj:
          Enzymes
          Erythrocytes Metabolism
          Funding Source
          Human
      ab: Characterizing the capabilities, key dependencies, and response to perturbations of genome-scale metabolic networks is a basic problem with important applications. A key question concerns the identification of the potentially most harmful reaction knockouts. The integration of combinatorial methods with sampling techniques to explore the space of viable flux states may provide crucial insights on this issue. We assess the replaceability of every metabolic conversion in the human red blood cell by enumerating the alternative paths from substrate to product, obtaining a complete map of he potential damage of single enzymopathies. Sampling the space of optimal steady state fluxes in the healthy and in the mutated cell reveals both correlations and complementarity between topologic and dynamical aspects.
      pubtype: Academic Journal
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        research
        tables/charts
        Journal Article
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    language: English
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