Biomaterials modulate interleukin-8 and other inflammatory proteins during reepithelialization in cutaneous partial-thickness wounds in pigs.

Acute and chronic cutaneous wounds remain a clinical challenge that require a mechanistic understanding to advance treatment options. For example, the role of inflammatory mediators during wound healing is not completely understood. Biomimetic materials, such as an in situ photopolymerizable semi-in...

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Publicado en:Wound Repair & Regeneration Vol. 18; no. 5; pp. 486 - 499
Autores principales: Kleinbeck KR, Faucher LD, Kao WJ
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell Sep/Oct2010
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Sep/Oct2010
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      pub: Wiley-Blackwell
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        10.1111/j.1524-475X.2010.00609.x
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        atl: Biomaterials modulate interleukin-8 and other inflammatory proteins during reepithelialization in cutaneous partial-thickness wounds in pigs.
      aug:
        au:
          Kleinbeck KR
          Faucher LD
          Kao WJ
        affil: Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, Madison, Wisconsin
      sug:
        subj:
          Skin Injuries
          Wounds and Injuries Therapy
          Skin, Artificial
          Inflammation Physiopathology
          Interleukins Metabolism
          Animal Studies
          Swine
          Models, Biological
          Funding Source
          T-Tests
      ab: Acute and chronic cutaneous wounds remain a clinical challenge that require a mechanistic understanding to advance treatment options. For example, the role of inflammatory mediators during wound healing is not completely understood. Biomimetic materials, such as an in situ photopolymerizable semi-interpenetrating network (sIPN) derived from extracellular matrix components, show great potential in improving healing through the delivery of therapeutic agents and the function as a temporary tissue scaffold. In this study, we characterized the temporal profile of porcine cutaneous partial-thickness wound healing in response to Xeroform™ and sIPN treatment via histological and inflammatory protein analyses in epidermal, remodeling dermal, and dermal regions. Generally, interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-10, IL-12p70, interferon-γ, and tumor necrosis factor-α, but not IL-8, were expressed in the epidermis and remodeling dermis in a time course that followed the progression of epidermal maturation in response to both treatments. Differences in cellularity and protein expression were observed between treatments in a time- and region-dependent manner. In particular, the healing response to sIPN exemplified a potentially key relationship between IL-8 expression and reepithelialization. These results provide insights into the expression of inflammatory mediators and the time course of cutaneous healing and the capacity for biomaterials to further modulate this relationship.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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