Left Ventricular Extracellular Matrix Remodeling in Dogs with Right Ventricular Apical Pacing.
Left Ventricular Remodeling in Pacing Dogs. Introduction: Right ventricle (RV) apical pacing is associated with increased incidence of heart failure due to left ventricle (LV) desynchronization. We aim to investigate extracellular matrix (ECM) remodeling of the LV in dogs with atria-sensed RV apical...
| Publicado en: | Journal of Cardiovascular Electrophysiology Vol. 21; no. 10; pp. 1142 - 1150 |
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| Autores principales: | , , , , |
| Formato: | research Journal Article |
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Wiley-Blackwell
Oct2010
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105109720&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105109720 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10453873 GSB jtl: Journal of Cardiovascular Electrophysiology issn: 10453873 maglogo: Y pubinfo: dt: Oct2010 vid: 21 iid: 10 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 105109720 54287265 10.1111/j.1540-8167.2010.01765.x NLM20384649 105109720 ppf: 1142 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Left Ventricular Extracellular Matrix Remodeling in Dogs with Right Ventricular Apical Pacing. aug: au: LIN J LAI L LIN C CHOU N CHIU C affil: *Department of Internal Medicine, Keelung Hospital, Department of Health, Executive Yuan, Keelung,Taiwan sug: subj: Pacemaker, Artificial Heart Failure Physiopathology Pacemaker, Artificial Adverse Effects Animal Studies Dogs Taiwan Equipment and Supplies Data Analysis Software Heart Pathology Blotting, Western Electrocardiography Echocardiography T-Tests Fluorescent Antibody Technique ab: Left Ventricular Remodeling in Pacing Dogs. Introduction: Right ventricle (RV) apical pacing is associated with increased incidence of heart failure due to left ventricle (LV) desynchronization. We aim to investigate extracellular matrix (ECM) remodeling of the LV in dogs with atria-sensed RV apical pacing. Methods and Results: Dogs with pacemakers underwent AV nodal ablation. After 12 weeks of atria-sensed obligatory RV pacing, LVs were separated into septum and lateral wall for analysis. Zymographic activity, including matrix metalloproteinase-2 (MMP-2), MMP-9, tissue inhibitors of metalloproteinase-1 (TIMP-1), TIMP-3, collagen transcript expression, and histology were examined in opposite portions of the LV to identify possible ECM remodeling changes by RV apical pacing. Compared with sham-operated dogs, increased interstitial fibrosis and fragmentation of myofibrils was found in the LV lateral wall in the pacing group. Collagen type II mRNA showed a significant 2-fold increase in the LV lateral wall in the pacing group. Although collagen type I mRNA was increased, the difference was not significant. Zymography demonstrated MMP-9 activity was enhanced in both the LV lateral wall and septum in the pacing group, but MMP-2 activity was enhanced in the LV lateral wall. Immunfluorescence stain confirmed the activation of MMP-2 and MMP-9 in the LV lateral wall in the pacing group. Protein expression of TIMP-1 and TIMP-3 showed regional differences in the pacing group and both proteins were increased in the LV lateral wall. Conclusion: LV dyssynchrony by RV apical pacing elicits heterogeneous ECM remodeling in the LV. These findings assist in the elucidation of the pathophysiology of LV desynchronization. (J Cardiovasc Electrophysiol, Vol. 21, pp. 1142-1149) pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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