Acute stress alters transcript expression pattern and reduces processing of proBDNF to mature BDNF in Dicentrarchus labrax.
Background: Stress involves alterations of brain functioning that may precipitate to mood disorders. The neurotrophin Brain Derived Neurotrophic Factor (BDNF) has recently been involved in stress-induced adaptation. BDNF is a key regulator of neuronal plasticity and adaptive processes. Regulation of...
| Publicado en: | BMC Neuroscience Vol. 11; pp. 4 - 5 |
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| Autores principales: | , , , , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
BioMed Central
2010
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105124995&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105124995 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14712202 1CI8 jtl: BMC Neuroscience issn: 14712202 maglogo: N pubinfo: dt: 2010 vid: 11 pid: 24147 pub: BioMed Central artinfo: ui: 105124995 NLM20074340 2010571721 10.1186/1471-2202-11-4 NLM20074340 PMC2829032 105124995 ppf: 4 ppct: 1 formats: tig: atl: Acute stress alters transcript expression pattern and reduces processing of proBDNF to mature BDNF in Dicentrarchus labrax. aug: au: Tognoli C Rossi F Di Cola F Baj G Tongiorgi E Terova G Saroglia M Bernardini G Gornati R Tognoli, Chiara Rossi, Federica Di Cola, Francesco Baj, Gabriele Tongiorgi, Enrico Terova, Genciana Saroglia, Marco Bernardini, Giovanni Gornati, Rosalba affil: Department of Biotechnology and Molecular Science, University of Insubria, Varese, Italy sug: subj: Genes Nerve Tissue Proteins Nerve Tissue Proteins Metabolism Stress, Psychological Stress, Psychological Metabolism Acute Disease Amino Acids Animal Studies Biochemical Phenomena Biological Markers Metabolism Documentation Fish Models, Biological Nucleotides Proteins Proteins Metabolism RNA RNA Metabolism Sensitivity and Specificity Stress, Psychological Diagnosis ab: Background: Stress involves alterations of brain functioning that may precipitate to mood disorders. The neurotrophin Brain Derived Neurotrophic Factor (BDNF) has recently been involved in stress-induced adaptation. BDNF is a key regulator of neuronal plasticity and adaptive processes. Regulation of BDNF is complex and may reflect not only stress-specific mechanisms but also hormonal and emotional responses. For this reason we used, as an animal model of stress, a fish whose brain organization is very similar to that of higher vertebrates, but is generally considered free of emotional reactions.Results: We provide a comprehensive characterization of BDNF gene in the Dicentrarchus labrax and its transcriptional, translational and post-translational regulation following acute stress. While total BDNF mRNA levels are unchanged, BDNF transcripts 1c and 1d resulted down regulated after acute stress. Acute stress induces also a significant increase in proBDNF levels and reduction in mature BDNF suggesting altered regulation of proBDNF proteolytic processing. Notably, we provide here the first evidence that fishes possess a simplified proteolytic regulation of BDNF since the pro28Kda form, generated by the SKI-1 protease in mammals, is absent in fishes because the cleavage site has first emerged in reptilians. Finally, we show that the proBDNF/totBDNF ratio is a highly predictive novel quantitative biomarker to detect stress in fishes with sensitivity = 100%, specificity = 87%, and Negative Predictive Value = 100%.Conclusion: The high predictivity of proBDNF/totBDNF ratio for stress in lower vertebrates indicates that processing of BDNF is a central mechanism in adaptation to stress and predicts that a similar regulation of pro/mature BDNF has likely been conserved throughout evolution of vertebrates from fish to man. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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