A comparison amongst three rapid urease tests to diagnose Helicobacter pylori infection in 375 consecutive dyspeptic.

Rapid diagnostic tools for Helicobacter pylori are important in endoscopy. To assess the accuracy of a new 5-min rapid urease test (UFT300, ABS Cernusco, sul Naviglio, Italy) compared with the 1-h Pyloritek (Serim Laboratories, Elkhart, IN) and the 24-h CLO test (Kimberly-Clark Ballard Medical Produ...

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Publicado en:Internal & Emergency Medicine Vol. 5; no. 1; pp. 41 - 48
Autores principales: Vaira D, Gatta L, Ricci C, Perna F, Saracino I, Fiorini G, Castelli V, Holton J, Vaira, Dino, Gatta, Luigi, Ricci, Chiara, Perna, Federico, Saracino, Ilaria, Fiorini, Giulia, Castelli, Valentina, Holton, John
Formato: research Journal Article
Publicado: Springer Nature Feb2010
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Feb2010
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      pub: Springer Nature
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        10.1007/s11739-009-0344-2
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        atl: A comparison amongst three rapid urease tests to diagnose Helicobacter pylori infection in 375 consecutive dyspeptic.
      aug:
        au:
          Vaira D
          Gatta L
          Ricci C
          Perna F
          Saracino I
          Fiorini G
          Castelli V
          Holton J
          Vaira, Dino
          Gatta, Luigi
          Ricci, Chiara
          Perna, Federico
          Saracino, Ilaria
          Fiorini, Giulia
          Castelli, Valentina
          Holton, John
        affil: Department of Internal Medicine and Gastroenterology, S. Orsola Hospital, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy
      sug:
        subj:
          Dyspepsia Microbiology
          Helicobacter Infections Diagnosis
          Reagent Kits, Diagnostic
          Urease Analysis
          Adult
          Aged
          Breath Tests
          False Negative Results
          Female
          Gastroscopy
          Helicobacter Infections Pathology
          Human
          Male
          Middle Age
          Prospective Studies
          Sensitivity and Specificity
          Urea Analysis
          Adult: 19-44 years
          Aged: 65+ years
          Middle Aged: 45-64 years
          Female
          Male
      ab: Rapid diagnostic tools for Helicobacter pylori are important in endoscopy. To assess the accuracy of a new 5-min rapid urease test (UFT300, ABS Cernusco, sul Naviglio, Italy) compared with the 1-h Pyloritek (Serim Laboratories, Elkhart, IN) and the 24-h CLO test (Kimberly-Clark Ballard Medical Products, Roswell, GA), consecutive dyspeptic patients referred to our unit for endoscopy were prospectively studied. All patients underwent a 13C-urea-breath test, histology and the UFT300 (ABS; Cernusco, sul Naviglio, Italy). Two additional rapid urease tests were performed. Patients were deemed infected when both 13C-UBT and histology were positive. Rapid urease tests were read at 1, 5, and 60 min, respectively. Of the 375 enrolled patients, 45.3% were infected with H. pylori. The sensitivity of the new 5-min rapid urease test 300 was 90.3, 94.5, and 96.2% at 1, 5, and 60 min, respectively (specificity 100%). The Pyloritek and the new 5-min rapid urease test were comparable, but the CLO test was not reliable at 5 and 60 min. In conclusion, the new 5-min rapid urease test is comparable to the Pyloritek test, but the CLO test is significantly less sensitive at early time points. Reading the test results at 1 min may increase false-negative results with decreasing sensitivity.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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