Direct immunofluorescence of skin biopsy: Perspective of an immunopathologist.
Background: By direct immunofluorescence (DIF), presence of immune complexes in the skin biopsy at various locations such as the dermo-epidermal junction, dermal blood vessels, etc. help to arrive at a diagnosis. Aims: (1) To study the role of DIF in confirmation or exclusion of diseases involving s...
| Publicado en: | Indian Journal of Dermatology, Venereology & Leprology Vol. 76; no. 2; pp. 150 - 158 |
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| Autores principales: | , , , , |
| Formato: | Journal Article |
| Publicado: |
Scientific Scholar LLC
Mar/Apr2010
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105144537&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105144537 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03786323 SV4 jtl: Indian Journal of Dermatology, Venereology & Leprology issn: 03786323 maglogo: N pubinfo: dt: Mar/Apr2010 vid: 76 iid: 2 pid: 56013 pub: Scientific Scholar LLC place: Pittsford, New York artinfo: ui: 105144537 2010593649 10.4103/0378-6323.60561 NLM20228544 105144537 ppf: 150 ppct: 8 formats: tig: atl: Direct immunofluorescence of skin biopsy: Perspective of an immunopathologist. aug: au: Minz RW Chhabra S Singh S Radotra BD Kumar B affil: Department of Immunopathology, Postgraduate Institute of Medical Education and Research, Chandigarh, India. sug: subj: Skin Skin Pathology Skin Diseases Immunology Skin Diseases Pathology Adolescence Adult Biopsy Child Child, Preschool Female Fluorescent Antibody Technique Methods Immunologic Techniques Male Middle Age Skin Diseases Diagnosis Young Adult Adolescent: 13-18 years Adult: 19-44 years Child: 6-12 years Child, Preschool: 2-5 years Middle Aged: 45-64 years Female Male ab: Background: By direct immunofluorescence (DIF), presence of immune complexes in the skin biopsy at various locations such as the dermo-epidermal junction, dermal blood vessels, etc. help to arrive at a diagnosis. Aims: (1) To study the role of DIF in confirmation or exclusion of diseases involving skin vis-à-vis histopathology and clinical diagnosis, (2) to describe the annual spectrum of dermatologic conditions that present to a tertiary referral center and require DIF examination of skin biopsy for confirmation of diagnosis. Methods: A total of 267 biopsies received over a period of 16 months in the Department of Immunopathology were analyzed along with clinical and histopathological details and the correlation between them was studied. Results: DIF was positive in 204 skin biopsies. Of these, 127 biopsies showed good clinico-immuno-histopathological correlation. In 10 cases, only DIF could clinch the diagnosis. In another nine cases, immune deposits were noted, which were unexpected in light of clinical and histopathological diagnosis. The most common skin involvement was seen in vasculitides. DIF was, however, non-contributory in lesions like erythema multiformè, post Kala-azar dermal leishmaniasis, sarcoidosis, lupus vulgaris, pyoderma gangrenosum and prurigo nodularis. Conclusion: The DIF of skin in conjunction with histopathology gives the best diagnostic yield. It is invaluable in confirming the diagnosis of small vessel vasculitides and bullous lesions of skin and can be used as an additional tool to pinpoint the diagnosis of systemic and localized autoimmune diseases involving the skin. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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