Phenotypic and genetic characterization of antimicrobial profiles of Helicobacter pylori strains in Cuba.

The study evaluated the antibiotic resistance patterns of Helicobacter pylori strains against metronidazole and clarithromycin in a hospital in Havana, Cuba. Eighty-five percent, 22.5%, and 10% of 40 H. pylori strains investigated were resistant to metronidazole, ciprofloxacin, and clarithromycin re...

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Publicado en:Journal of Health, Population & Nutrition Vol. 28; no. 2; pp. 124 - 130
Autores principales: Llanes R, Soria C, Nagashima S, Kobayashi N, Gala A, Guzmán D, Feliciano O, Valdés L, Gutiérrez O, Fernández H, Llop A, Wada A, Llanes, Rafael, Soria, Claudia, Nagashima, Shigeo, Kobayashi, Nobumichi, Gala, Angela, Guzmán, Daymi, Feliciano, Onelkis, Valdés, Lidunka
Formato: research Journal Article
Publicado: BioMed Central Apr2010
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Apr2010
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        atl: Phenotypic and genetic characterization of antimicrobial profiles of Helicobacter pylori strains in Cuba.
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          Llanes R
          Soria C
          Nagashima S
          Kobayashi N
          Gala A
          Guzmán D
          Feliciano O
          Valdés L
          Gutiérrez O
          Fernández H
          Llop A
          Wada A
          Llanes, Rafael
          Soria, Claudia
          Nagashima, Shigeo
          Kobayashi, Nobumichi
          Gala, Angela
          Guzmán, Daymi
          Feliciano, Onelkis
          Valdés, Lidunka
        affil: Microbiology, Clinical and Epidemiology Branch, Institute Pedro Kouri, Havana, Cuba
      sug:
        subj:
          Antibiotics Pharmacodynamics
          Antiinfective Agents Pharmacodynamics
          Helicobacter Pylori Drug Effects
          Helicobacter Pylori
          Mutation
          Adult
          Age Factors
          Aged
          Amoxicillin Pharmacodynamics
          Ciprofloxacin Pharmacodynamics
          Clarithromycin Pharmacodynamics
          Cuba
          Drug Resistance, Microbial
          Female
          Helicobacter Pylori Classification
          Human
          Male
          Metronidazole Pharmacodynamics
          Microbial Culture and Sensitivity Tests Methods
          Middle Age
          Polymerase Chain Reaction Methods
          Sequence Analysis Methods
          Sex Factors
          Immunity
          Tetracycline Pharmacodynamics
          Young Adult
          Adult: 19-44 years
          Aged: 65+ years
          Middle Aged: 45-64 years
          Female
          Male
      ab: The study evaluated the antibiotic resistance patterns of Helicobacter pylori strains against metronidazole and clarithromycin in a hospital in Havana, Cuba. Eighty-five percent, 22.5%, and 10% of 40 H. pylori strains investigated were resistant to metronidazole, ciprofloxacin, and clarithromycin respectively but all were susceptible to amoxicillin and tetracycline. RdxA truncation was found only in metronidazole-resistant strains. In such strains, reported are eight and two novel mutations in the rdxA and frxA genes respectively. Two-point mutations in the 23S rRNA genes of clarithromycin-resistant strains were detected. A high prevalence of metronidazole resistance was found in Cuban H. pylori strains. Mutations in the rdxA gene may contribute more significantly than frxA gene to the high level of resistance to metronidazole. This study supports the need to continue monitoring the antibiotic susceptibility in H. pylori in Cuba to guide the treatment of such infection.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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