Stratification of antigen-presenting cells within the normal cornea.
The composition and location of professional antigen presenting cells (APC) varies in different mucosal surfaces. The cornea, long considered an immune-privileged tissue devoid of APCs, is now known to host a heterogeneous network of bone marrow-derived cells. Here, we utilized transgenic mice that...
| Publicado en: | Ophthalmology & Eye Diseases no. 1; pp. 45 - 55 |
|---|---|
| Autores principales: | , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Sage Publications Inc.
2009
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=105202634&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 105202634 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11791721 B3LA jtl: Ophthalmology & Eye Diseases issn: 11791721 maglogo: Y pubinfo: dt: 2009 iid: 1 pid: 344 pub: Sage Publications Inc. place: Thousand Oaks, California artinfo: ui: 105202634 105202634 2010656922 105202634 ppf: 45 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Stratification of antigen-presenting cells within the normal cornea. aug: au: Knickelbein JE Watkins SC McMenamin PG Hendricks RL affil: Medical Scientist Training Program, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA sug: subj: Antigen-Presenting Cells Immunology Cornea Physiology Dendritic Cells Immunology Analysis of Variance Animal Studies Data Analysis Software Funding Source Immunohistochemistry Mice Microscopy Methods Phenotype Post Hoc Analysis ab: The composition and location of professional antigen presenting cells (APC) varies in different mucosal surfaces. The cornea, long considered an immune-privileged tissue devoid of APCs, is now known to host a heterogeneous network of bone marrow-derived cells. Here, we utilized transgenic mice that express enhanced green fluorescent protein (EGFP) from the CD11c promoter (pCD11c) in conjunction with immunohistochemical staining to demonstrate an interesting stratification of APCs within non-inflamed murine corneas. pCD11c+ dendritic cells (DCs) reside in the basal epithelium, seemingly embedded in the basement membrane. Most DCs express MHC class II on at least some dendrites, which extend up to 50 μm in length and traverse up 20 μm tangentially towards the apical surface of the epithelium. The DC density diminishes from peripheral to central cornea. Beneath the DCs and adjacent to the stromal side of the basement membrane reside pCD11c-CD11b+ putative macrophages that express low levels of MHC class II. Finally, MHC class II-pCD11c-CD11b+ cells form a network throughout the remainder of the stroma. This highly reproducible stratification of bone marrow-derived cells is suggestive of a progression from an APC function at the exposed corneal surface to an innate immune barrier function deeper in the stroma. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|